Breeding with rare defective alleles (BRDA) a natural Populus nigra HCT mutant with modified lignin as a case study

Breeding with rare defective alleles (BRDA) : a natural Populus nigra HCT mutant with modified lignin as a case study

© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 198(2013), 3 vom: 26. Mai, Seite 765-776
1. Verfasser: Vanholme, Bartel (VerfasserIn)
Weitere Verfasser: Cesarino, Igor, Goeminne, Geert, Kim, Hoon, Marroni, Fabio, Van Acker, Rebecca, Vanholme, Ruben, Morreel, Kris, Ivens, Bart, Pinosio, Sara, Morgante, Michele, Ralph, John, Bastien, Catherine, Boerjan, Wout
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 4-coumaroylshikimic acid Shikimic Acid 29MS2WI2NU Lignin 9005-53-2 Acyltransferases EC 2.3.-
Beschreibung
Zusammenfassung:© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
Next-generation (NG) sequencing in a natural population of Populus nigra revealed a mutant with a premature stop codon in the gene encoding hydroxycinnamoyl-CoA : shikimate hydroxycinnamoyl transferase1 (HCT1), an essential enzyme in lignin biosynthesis. The lignin composition of P. nigra trees homozygous for the defective allele was compared with that of heterozygous trees and trees without the defective allele. The lignin was characterized by phenolic profiling, lignin oligomer sequencing, thioacidolysis and NMR. In addition, HCT1 was heterologously expressed for activity assays and crosses were made to introduce the mutation in different genetic backgrounds. HCT1 converts p-coumaroyl-CoA into p-coumaroyl shikimate. The mutant allele, PnHCT1-Δ73, encodes a truncated protein, and trees homozygous for this recessive allele have a modified lignin composition characterized by a 17-fold increase in p-hydroxyphenyl units. Using the lignin pathway as proof of concept, we illustrated that the capture of rare defective alleles is a straightforward approach to initiate reverse genetics and accelerate tree breeding. The proposed breeding strategy, called 'breeding with rare defective alleles' (BRDA), should be widely applicable, independent of the target gene or the species
Beschreibung:Date Completed 25.11.2013
Date Revised 08.04.2022
published: Print-Electronic
GENBANK: JF693234
CommentIn: New Phytol. 2013 May;198(3):635-7. - PMID 23577594
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.12179