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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2013.01.006
|2 doi
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|a pubmed24n1253.xml
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|a (DE-627)NLM225093413
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|a (NLM)23416492
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|a (PII)S0981-9428(13)00025-9
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Luo, Tao
|e verfasserin
|4 aut
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| 245 |
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|a Virus-induced gene silencing of pea CHLI and CHLD affects tetrapyrrole biosynthesis, chloroplast development and the primary metabolic network
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.09.2013
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|a Date Revised 09.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier Masson SAS. All rights reserved.
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|a The first committed and highly regulated step of chlorophyll biosynthesis is the insertion of Mg(2+) into protoporphyrin IX, which is catalyzed by Mg chelatase that consists of CHLH, CHLD and CHLI subunits. In this study, CHLI and CHLD genes were suppressed by virus-induced gene silencing (VIGS-CHLI and VIGS-CHLD) in pea (Pisum sativum), respectively. VIGS-CHLI and VIGS-CHLD plants both showed yellow leaf phenotypes with the reduced Mg chelatase activity and the inactivated synthesis of 5-aminolevulinic acid. The lower chlorophyll accumulation correlated with undeveloped thylakoid membranes, altered chloroplast nucleoid structure, malformed antenna complexes and compromised photosynthesis capacity in the yellow leaf tissues of the VIGS-CHLI and VIGS-CHLD plants. Non-enzymatic antioxidant contents and the activities of antioxidant enzymes were altered in response to enhanced accumulation of reactive oxygen species (ROS) in the chlorophyll deficient leaves of VIGS-CHLI and VIGS-CHLD plants. Furthermore, the results of metabolite profiling indicate a tight correlation between primary metabolic pathways and Mg chelatase activity. We also found that CHLD induces a feedback-regulated change of the transcription of photosynthesis-associated nuclear genes. CHLD and CHLI silencing resulted in a rapid reduction of photosynthetic proteins. Taken together, Mg chelatase is not only a key regulator of tetrapyrrole biosynthesis but its activity also correlates with ROS homeostasis, primary interorganellar metabolism and retrograde signaling in plant cells
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a Tetrapyrroles
|2 NLM
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|a Luo, Sha
|e verfasserin
|4 aut
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|a Araújo, Wagner L
|e verfasserin
|4 aut
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| 700 |
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|a Schlicke, Hagen
|e verfasserin
|4 aut
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| 700 |
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|a Rothbart, Maxi
|e verfasserin
|4 aut
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| 700 |
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|a Yu, Jing
|e verfasserin
|4 aut
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| 700 |
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|a Fan, Tingting
|e verfasserin
|4 aut
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| 700 |
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|a Fernie, Alisdair R
|e verfasserin
|4 aut
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| 700 |
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|a Grimm, Bernhard
|e verfasserin
|4 aut
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| 700 |
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|a Luo, Meizhong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 65(2013) vom: 01. Apr., Seite 17-26
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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| 773 |
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|g volume:65
|g year:2013
|g day:01
|g month:04
|g pages:17-26
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|u http://dx.doi.org/10.1016/j.plaphy.2013.01.006
|3 Volltext
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