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231224s2013 xx |||||o 00| ||eng c |
| 024 |
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|a 10.1021/la304548m
|2 doi
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|a pubmed24n0750.xml
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|a DE-627
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Horiguchi, Yukichi
|e verfasserin
|4 aut
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| 245 |
1 |
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|a High-performance surface acoustic wave immunosensing system on a PEG/aptamer hybridized surface
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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| 500 |
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|a Date Completed 06.01.2014
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|a Date Revised 03.12.2018
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Label-free immunoassay systems have the advantages of procedural simplicity and a low construction cost of surfaces for immunosensing. When label-free immunoassay systems are considered, the nonspecific adsorption of unwanted materials should be eliminated unless it aids in the detection of error. PEG is well-known as a blocking agent for the prevention of the adsorption of nonspecific binding materials when coimmobilized with ligands for targets such as antibodies and oligonucleotides. The construction strategy for PEG/ligand coimmobilized surfaces is an important point in the preparation of a high-performance assays because the physiological condition of the ligand depends strongly on its interaction with the PEG chain. In this report, we investigate the interaction between thrombin and a thrombin-binding aptamer (TBA) on a PEG/TBA coimmobilized surface by using a shear horizontal surface acoustic wave (SAW) sensor. The thrombin-TBA binding property shows remarkable differences with changes in the PEG density and the distance from the gold surface to the aptamer
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA Primers
|2 NLM
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| 650 |
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7 |
|a Polyethylene Glycols
|2 NLM
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| 650 |
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|a 3WJQ0SDW1A
|2 NLM
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| 700 |
1 |
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|a Miyachi, Seigo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Nagasaki, Yukio
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 29(2013), 24 vom: 18. Juni, Seite 7369-76
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:29
|g year:2013
|g number:24
|g day:18
|g month:06
|g pages:7369-76
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|u http://dx.doi.org/10.1021/la304548m
|3 Volltext
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