Implementation and performance of SIBYLS : a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source
The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique amon...
| Publié dans: | Journal of applied crystallography. - 1998. - 46(2013), Pt 1 vom: 01. Feb., Seite 1-13 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
2013
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| Accès à la collection: | Journal of applied crystallography |
| Sujets: | Journal Article SIBYLS macromolecular crystallography (MX) small-angle X-ray scattering (SAXS) synchrotron beamlines |
| Résumé: | The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B(4)C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources |
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| Description: | Date Revised 06.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 0021-8898 |