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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2012.12.011
|2 doi
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|a pubmed25n0749.xml
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|a (DE-627)NLM224883828
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|a (NLM)23394787
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|a (PII)S0176-1617(13)00029-1
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Martínez-Andújar, Cristina
|e verfasserin
|4 aut
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|a Response to nitrate/ammonium nutrition of tomato (Solanum lycopersicum L.) plants overexpressing a prokaryotic NH4(+)-dependent asparagine synthetase
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|c 2013
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 10.09.2013
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2013 Elsevier GmbH. All rights reserved.
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|a Nitrogen availability is an important limiting factor for plant growth. Although NH4(+) assimilation is energetically more favorable than NO3(-), it is usually toxic for plants. In order to study if an improved ammonium assimilatory metabolism could increase the plant tolerance to ammonium nutrition, tomato (Solanum lycopersicum L. cv P-73) plants were transformed with an NH4(+)-dependent asparagine synthetase (AS-A) gene from Escherichia coli (asnA) under the control of a PCpea promoter (pea isolated constitutive promotor). Homozygous (Hom), azygous (Az) asnA and wild type (WT) plants were grown hydroponically for 6 weeks with normal Hoagland nutrition (NO3(-)/NH4(+)=6/0.5) and high ammonium nutrition (NO3(-)/NH4(+)=3.5/3). Under Hoagland's conditions, Hom plants produced 40-50% less biomass than WT and Az plants. However, under NO3(-)/NH4(+)=3.5/3 the biomass of Hom was not affected while it was reduced by 40-70% in WT and Az plants compared to Hoagland, respectively. The Hom plants accumulated 1.5-4 times more asparagine, glycine, serine and soluble proteins and registered higher glutamine synthetase (GS) and glutamate synthase (GOGAT) activities in the light-adapted leaves than the other genotypes, but had similar NH4(+) and NO3(-) levels in all conditions. In the dark-adapted leaves, a protein catabolism occurred in the Hom plants with a concomitant 25-40% increase in organic acid concentration, while asparagine accumulation registered the highest values. The aforementioned processes might be responsible for a positive energetic balance as regards the futile cycle of the transgenic protein synthesis and catabolism. This explains growth penalty under standard nutrition and growth stability under NO3(-)/NH4(+)=3.5/3, respectively
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|a Journal Article
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|a Amino Acids
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|a Bacterial Proteins
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|a Carbohydrates
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|a Carboxylic Acids
|2 NLM
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|a Nitrates
|2 NLM
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|a Quaternary Ammonium Compounds
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|a Chlorophyll
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|a 1406-65-1
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|a Asparagine
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|a 7006-34-0
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|a Aspartate-Ammonia Ligase
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|a EC 6.3.1.1
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|a Potassium
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|a RWP5GA015D
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|a Ghanem, Michel Edmond
|e verfasserin
|4 aut
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|a Albacete, Alfonso
|e verfasserin
|4 aut
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|a Pérez-Alfocea, Francisco
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 170(2013), 7 vom: 01. Mai, Seite 676-87
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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|g volume:170
|g year:2013
|g number:7
|g day:01
|g month:05
|g pages:676-87
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|u http://dx.doi.org/10.1016/j.jplph.2012.12.011
|3 Volltext
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