Thermospermine catabolism increases Arabidopsis thaliana resistance to Pseudomonas viridiflava

This work investigated the roles of the tetraamine thermospermine (TSpm) by analysing its contribution to Arabidopsis basal defence against the biotrophic bacterium Pseudomonas viridiflava. The participation of polyamine oxidases (PAOs) in TSpm homeostasis and TSpm-mediated defence was also investig...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:	Journal of experimental botany. - 1985. - 64(2013), 5 vom: 19. März, Seite 1393-402
1. Verfasser:	Marina, María (VerfasserIn)
Weitere Verfasser:	Sirera, Francisco Vera, Rambla, José L, Gonzalez, María E, Blázquez, Miguel A, Carbonell, Juan, Pieckenstain, Fernando L, Ruiz, Oscar A
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2013
Zugriff auf das übergeordnete Werk:	Journal of experimental botany
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Arabidopsis Proteins Enzyme Inhibitors Spermine 2FZ7Y3VOQX thermospermine 70862-11-2 Amine Oxidase (Copper-Containing) EC 1.4.3.21 mehr... Oxidoreductases Acting on CH-NH Group Donors EC 1.5.- Putrescine V10TVZ52E4

Beschreibung
Zusammenfassung:	This work investigated the roles of the tetraamine thermospermine (TSpm) by analysing its contribution to Arabidopsis basal defence against the biotrophic bacterium Pseudomonas viridiflava. The participation of polyamine oxidases (PAOs) in TSpm homeostasis and TSpm-mediated defence was also investigated. Exogenous supply of TSpm, as well as ectopic expression of the TSpm biosynthetic gene ACL5, increased Arabidopsis Col-0 resistance to P. viridiflava, while null acl5 mutants were less resistant than Col-0 plants. The above-mentioned increase in resistance was blocked by the PAO inhibitor SL-11061, thus demonstrating the participation of TSpm oxidation. Analysis of PAO genes expression in transgenic 35S::ACL5 and Col-0 plants supplied with TSpm suggests that PAO 1, 3, and 5 are the main PAOs involved in TSpm catabolism. In summary, TSpm exhibited the potential to perform defensive functions previously reported for its structural isomer Spm, and the relevance of these findings is discussed in the context of ACL5 expression and TSpm concentration in planta. Moreover, this work demonstrates that manipulation of TSpm metabolism modifies plant resistance to pathogens
Beschreibung:	Date Completed 27.08.2013 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1460-2431
DOI:	10.1093/jxb/ert012