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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/ert006
|2 doi
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|a pubmed24n1334.xml
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|a (DE-627)NLM224576267
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|a (NLM)23362300
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Begara-Morales, Juan C
|e verfasserin
|4 aut
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|a Protein tyrosine nitration in pea roots during development and senescence
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.07.2013
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|a Date Revised 18.03.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Protein tyrosine nitration is a post-translational modification mediated by reactive nitrogen species (RNS) that is associated with nitro-oxidative damage. No information about this process is available in relation to higher plants during development and senescence. Using pea plants at different developmental stages (ranging from 8 to 71 days), tyrosine nitration in the main organs (roots, stems, leaves, flowers, and fruits) was analysed using immunological and proteomic approaches. In the roots of 71-day-old senescent plants, nitroproteome analysis enabled the identification a total of 16 nitrotyrosine-immunopositive proteins. Among the proteins identified, NADP-isocitrate dehydrogenase (ICDH), an enzyme involved in the carbon and nitrogen metabolism, redox regulation, and responses to oxidative stress, was selected to evaluate the effect of nitration. NADP-ICDH activity fell by 75% during senescence. Analysis showed that peroxynitrite inhibits recombinant cytosolic NADP-ICDH activity through a process of nitration. Of the 12 tyrosines present in this enzyme, mass spectrometric analysis of nitrated recombinant cytosolic NADP-ICDH enabled this study to identify the Tyr392 as exclusively nitrated by peroxynitrite. The data as a whole reveal that protein tyrosine nitration is a nitric oxide-derived PTM prevalent throughout root development and intensifies during senescence
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Isoenzymes
|2 NLM
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|a Proteome
|2 NLM
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|a Peroxynitrous Acid
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|a 14691-52-2
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|a Nitric Oxide
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|a 31C4KY9ESH
|2 NLM
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|a Tyrosine
|2 NLM
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|a 42HK56048U
|2 NLM
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|a Isocitrate Dehydrogenase
|2 NLM
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|a EC 1.1.1.41
|2 NLM
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| 650 |
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|a isocitrate dehydrogenase (NADP+)
|2 NLM
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| 650 |
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|a EC 1.1.1.42
|2 NLM
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|a Superoxide Dismutase
|2 NLM
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|a EC 1.15.1.1
|2 NLM
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| 700 |
1 |
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|a Chaki, Mounira
|e verfasserin
|4 aut
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|a Sánchez-Calvo, Beatriz
|e verfasserin
|4 aut
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|a Mata-Pérez, Capilla
|e verfasserin
|4 aut
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|a Leterrier, Marina
|e verfasserin
|4 aut
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|a Palma, José M
|e verfasserin
|4 aut
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|a Barroso, Juan B
|e verfasserin
|4 aut
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| 700 |
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|a Corpas, Francisco J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 64(2013), 4 vom: 12. Feb., Seite 1121-34
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnns
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| 773 |
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|g volume:64
|g year:2013
|g number:4
|g day:12
|g month:02
|g pages:1121-34
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|u http://dx.doi.org/10.1093/jxb/ert006
|3 Volltext
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|h 1121-34
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