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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1021/la302212h
|2 doi
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|a pubmed25n0748.xml
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|a eng
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1 |
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|a Hentschel, Carsten
|e verfasserin
|4 aut
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|a AFM-based force spectroscopy on polystyrene brushes
|b effect of brush thickness on protein adsorption
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a Date Completed 29.07.2013
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|a Date Revised 25.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Herein we present a study on nonspecific binding of proteins at highly dense packed hydrophobic polystyrene brushes. In this context, an atomic force microscopy tip was functionalized with concanavalin A to perform single-molecule force spectroscopy measurements on polystyrene brushes with thicknesses of 10 and 60 nm, respectively. Polystyrene brushes with thickness of 10 nm show an almost two times stronger protein adsorption than brushes with a thickness of 60 nm: 72 pN for the thinner and 38 pN for the thicker layer, which is in qualitative agreement with protein adsorption studies conducted macroscopically by fluorescence microscopy
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Immobilized Proteins
|2 NLM
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|a Polystyrenes
|2 NLM
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|a Wagner, Hendrik
|e verfasserin
|4 aut
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1 |
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|a Smiatek, Jens
|e verfasserin
|4 aut
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|a Heuer, Andreas
|e verfasserin
|4 aut
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|a Fuchs, Harald
|e verfasserin
|4 aut
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|a Zhang, Xi
|e verfasserin
|4 aut
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|a Studer, Armido
|e verfasserin
|4 aut
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|a Chi, Lifeng
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 29(2013), 6 vom: 12. Feb., Seite 1850-6
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|x 1520-5827
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|g volume:29
|g year:2013
|g number:6
|g day:12
|g month:02
|g pages:1850-6
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|u http://dx.doi.org/10.1021/la302212h
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