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|a 10.1021/la304941a
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|a eng
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|a Viel, Pascal
|e verfasserin
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|a Versatile and nondestructive photochemical process for biomolecule immobilization
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|c 2013
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|a Text
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|a Date Completed 29.07.2013
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|a Date Revised 12.02.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Covalent immobilization of unmodified biological materials as proteins has been performed through a one-step and soft method. This process is based on a polyazidophenylene layer derived from the electroreduction of the parent salt 4-azidobenzenediazonium tetrafluoborate on gold substrates. The wavelength used (365 nm) for the photochemical grafting of a large variety of molecules as biomolecules is a key point to this nondestructive immobilization method. This simple process is also versatile and could be used for covalently binding a wide range of molecules such as polyethylene glycol moieties, for example. To validate this approach for biochip or microarray fabrication, a surface plasmon resonance imaging (SPRi) platform for immobilization of various antibody families was created by grafting G-protein through this process. This SPRi antibodies platform was tested with several consecutive cycles of antigen injections/regeneration steps without loss of activity
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|a Walter, Justine
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