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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1109/TUFFC.2013.2535
|2 doi
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|a DE-627
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|e rakwb
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|a eng
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|a Van Ruijssevelt, Lisbeth
|e verfasserin
|4 aut
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|a Observations on the viability of C6-glioma cells after sonoporation with low-intensity ultrasound and microbubbles
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 10.06.2013
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|a Date Revised 16.11.2017
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|a published: Print
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|a Citation Status MEDLINE
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|a Ultrasound (US) and microbubbles can be used to facilitate cellular uptake of drugs through a cavitationinduced enhancement of cell membrane permeability. The mechanism is, however, still incompletely understood. A direct contact between microbubbles and cell membrane is thought to be essential to create membrane perturbations lasting from seconds to minutes after US exposure of the cells. A recent study showed that the effect may even last up to 8 h after cavitation (with residual permeability up to 24 h after cavitation). In view of possible membrane damage, the purpose of this study was to further investigate the evolution of cell viability in the range of the 24-h temporal window. Furthermore, a description of the functional changes in tumor cells after US exposure was initiated to obtain a better understanding of the mechanism of membrane perturbation after sonication with microbubbles. Our results suggest that US does not reduce cell viability up to 24 h post-exposure. However, a perturbation of the entire cell population exposed to US was observed in terms of enzymatic activity and characteristics of the mitochondrial membrane. Furthermore, we demonstrated that US cavitation induces a transient loss of cell membrane asymmetry, resulting in phosphatidylserine exposure in the outer leaflet of the cell membrane
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1 |
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|a Smirnov, Pierre
|e verfasserin
|4 aut
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|a Yudina, Anna
|e verfasserin
|4 aut
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|a Bouchaud, Veronique
|e verfasserin
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|a Voisin, Pierre
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|a Moonen, Chrit
|e verfasserin
|4 aut
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|i Enthalten in
|t IEEE transactions on ultrasonics, ferroelectrics, and frequency control
|d 1986
|g 60(2013), 1 vom: 14. Jan., Seite 34-45
|w (DE-627)NLM098181017
|x 1525-8955
|7 nnns
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|g volume:60
|g year:2013
|g number:1
|g day:14
|g month:01
|g pages:34-45
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|u http://dx.doi.org/10.1109/TUFFC.2013.2535
|3 Volltext
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