Mechanisms of the ultrasound-mediated intracellular delivery of liposomes and dextrans

The mechanism involved in the ultrasoundenhanced intracellular delivery of fluorescein-isothiocyanate (FITC)-dextran (molecular weight 4 to 2000 kDa) and liposomes containing doxorubicin (Dox) was studied using HeLa cells and an ultrasound transducer at 300 kHz, varying the acoustic power. The cellu...

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Veröffentlicht in:IEEE transactions on ultrasonics, ferroelectrics, and frequency control. - 1986. - 60(2013), 1 vom: 14. Jan., Seite 21-33
1. Verfasser: Afadzi, Mercy (VerfasserIn)
Weitere Verfasser: Strand, Sabina P, Nilssen, Esben A, Måsøy, Svein-Erik, Johansen, Tonni F, Hansen, Rune, Angelsen, Bjørn A, de L Davies, Catharina
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:IEEE transactions on ultrasonics, ferroelectrics, and frequency control
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Dextrans Liposomes fluorescein isothiocyanate dextran Doxorubicin 80168379AG Fluorescein-5-isothiocyanate I223NX31W9
Beschreibung
Zusammenfassung:The mechanism involved in the ultrasoundenhanced intracellular delivery of fluorescein-isothiocyanate (FITC)-dextran (molecular weight 4 to 2000 kDa) and liposomes containing doxorubicin (Dox) was studied using HeLa cells and an ultrasound transducer at 300 kHz, varying the acoustic power. The cellular uptake and cell viability were measured using flow cytometry and confocal microscopy. The role of endocytosis was investigated by inhibiting clathrin- and caveolae-mediated endocytosis, as well as macropinocytosis. Microbubbles were found to be required during ultrasound treatment to obtain enhanced cellular uptake. The percentage of cells internalizing Dox and dextran increased with increasing mechanical index. Confocal images and flow cytometric analysis indicated that the liposomes were disrupted extracellularly and that released Dox was taken up by the cells. The percentage of cells internalizing dextran was independent of the molecular weight of dextrans, but the amount of the small 4-kDa dextran molecules internalized per cell was higher than for the other dextrans. The inhibition of endocytosis during ultrasound exposure resulted in a significant decrease in cellular uptake of dextrans. Therefore, the improved uptake of Dox and dextrans may be a result of both sonoporation and endocytosis
Beschreibung:Date Completed 10.06.2013
Date Revised 25.11.2016
published: Print
Citation Status MEDLINE
ISSN:1525-8955
DOI:10.1109/TUFFC.2013.2534