Poly(amido amine)-based mannose-glycodendrimers as multielectron redox probes for improving lectin sensing

An easy-to-prepare series of electroactive poly(amido amine) (PAMAM)-based dendrimers of generations G0 to G2 having mannopyranosylferrocenyl moieties in the periphery to detect carbohydrate-protein interactions is reported. The synthesis involved the functionalization of the PAMAM surface with azid...

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Veröffentlicht in:Langmuir : the ACS journal of surfaces and colloids. - 1992. - 29(2013), 4 vom: 29. Jan., Seite 1318-26
1. Verfasser: Martos-Maldonado, Manuel C (VerfasserIn)
Weitere Verfasser: Casas-Solvas, Juan M, Quesada-Soriano, Indalecio, García-Fuentes, Luis, Vargas-Berenguel, Antonio
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:Langmuir : the ACS journal of surfaces and colloids
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Dendrimers Ferrous Compounds Glycoconjugates Metallocenes PAMAM Starburst Concanavalin A 11028-71-0 Copper mehr... 789U1901C5 Mannose PHA4727WTP ferrocene U96PKG90JQ
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245 1 0 |a Poly(amido amine)-based mannose-glycodendrimers as multielectron redox probes for improving lectin sensing 
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520 |a An easy-to-prepare series of electroactive poly(amido amine) (PAMAM)-based dendrimers of generations G0 to G2 having mannopyranosylferrocenyl moieties in the periphery to detect carbohydrate-protein interactions is reported. The synthesis involved the functionalization of the PAMAM surface with azidomethylferrocenyl groups and subsequent coupling of mannoside units by the Cu(I)-catalyzed Huisgen reaction. The binding affinity of the series of electroactive glycodendrimers was studied by isothermal titration calorimetry (ITC) and differential pulse voltammetry (DPV). Upon complexation of the glycodendrimers conjugates with prototypical concanavalin A (Con A), voltammograms showed a decrease of the peak current. Such dendrimers showed a notable improvement of redox sensing abilities toward Con A when compared with mono- and divalent analogues, based on both the glycoside multivalent and ferrocene dendritic effects 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Ferrous Compounds  |2 NLM 
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650 7 |a Metallocenes  |2 NLM 
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650 7 |a 11028-71-0  |2 NLM 
650 7 |a Copper  |2 NLM 
650 7 |a 789U1901C5  |2 NLM 
650 7 |a Mannose  |2 NLM 
650 7 |a PHA4727WTP  |2 NLM 
650 7 |a ferrocene  |2 NLM 
650 7 |a U96PKG90JQ  |2 NLM 
700 1 |a Casas-Solvas, Juan M  |e verfasserin  |4 aut 
700 1 |a Quesada-Soriano, Indalecio  |e verfasserin  |4 aut 
700 1 |a García-Fuentes, Luis  |e verfasserin  |4 aut 
700 1 |a Vargas-Berenguel, Antonio  |e verfasserin  |4 aut 
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