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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12012
|2 doi
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|a pubmed25n0746.xml
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|a (DE-627)NLM223807974
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|a (NLM)23278320
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Jackson, Dorothy E
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Evaluation of fixation time using Diff-Quik for staining of canine mast cell tumor aspirates
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.08.2013
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|a Date Revised 20.10.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Mast cell tumors are the most common cutaneous tumor in the dog and are often diagnosed via fine-needle aspiration and cytology. Many veterinary practices use Diff-Quik stain for these cases because it is easy to use and provides rapid results. Anecdotal reports suggest that Diff-Quik does not stain mast cell tumor granules well and that increased duration of fixation time can improve staining quality; however, this has not been prospectively evaluated
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|a OBJECTIVES: The aim of this study was to determine if varying fixation time would affect the staining quality of mast cell granules using the Diff-Quik stain. The null hypothesis was that there would be no difference in the staining of the granules based on duration of time in the fixation solution
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|a METHODS: Fine-needle aspirates of cutaneous mast cell tumors were obtained from 21 dogs and distributed on multiple slides. These slides were then stained in Diff-Quik at varying fixation times (ie, 5 seconds, 30 seconds, 1 minute, 2 minutes). One slide was stained with modified Wright stain as a control. Mast cell staining quality was evaluated either by blinded clinicopathologic review (n = 12) or by computer analysis of photomicrographs (n = 6). Results were compared with histopathologic grade
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|a RESULTS: There was no difference in staining quality among groups
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|a CONCLUSIONS: Alteration in fixation time using Diff-Quik does not improve staining characteristics of mast cell tumors
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|a Journal Article
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|a Azure Stains
|2 NLM
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| 650 |
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|a Diff Quik
|2 NLM
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| 650 |
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|a Xanthenes
|2 NLM
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| 650 |
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|a Methylene Blue
|2 NLM
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|a T42P99266K
|2 NLM
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| 700 |
1 |
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|a Selting, Kim A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Spoor, Melanie S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Henry, Carolyn J
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wiedmeyer, Charles E
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 42(2013), 1 vom: 02. März, Seite 99-102
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
1 |
8 |
|g volume:42
|g year:2013
|g number:1
|g day:02
|g month:03
|g pages:99-102
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| 856 |
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|u http://dx.doi.org/10.1111/vcp.12012
|3 Volltext
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|d 42
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|h 99-102
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