|
|
|
|
| LEADER |
01000caa a22002652 4500 |
| 001 |
NLM223579246 |
| 003 |
DE-627 |
| 005 |
20250214185402.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2013 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1107/S0909049512048303
|2 doi
|
| 028 |
5 |
2 |
|a pubmed25n0745.xml
|
| 035 |
|
|
|a (DE-627)NLM223579246
|
| 035 |
|
|
|a (NLM)23254651
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Warkentin, Matthew
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Global radiation damage
|b temperature dependence, time dependence and how to outrun it
|
| 264 |
|
1 |
|c 2013
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 13.06.2013
|
| 500 |
|
|
|a Date Revised 17.05.2024
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a A series of studies that provide a consistent and illuminating picture of global radiation damage to protein crystals, especially at temperatures above ∼200 K, are described. The radiation sensitivity shows a transition near 200 K, above which it appears to be limited by solvent-coupled diffusive processes. Consistent with this interpretation, a component of global damage proceeds on timescales of several minutes at 180 K, decreasing to seconds near room temperature. As a result, data collection times of order 1 s allow up to half of global damage to be outrun at 260 K. Much larger damage reductions near room temperature should be feasible using larger dose rates delivered using microfocused beams, enabling a significant expansion of structural studies of proteins under more nearly native conditions
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, N.I.H., Extramural
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
|
| 650 |
|
7 |
|a Plant Proteins
|2 NLM
|
| 650 |
|
7 |
|a Proteins
|2 NLM
|
| 650 |
|
7 |
|a thaumatin protein, plant
|2 NLM
|
| 650 |
|
7 |
|a 53850-34-3
|2 NLM
|
| 700 |
1 |
|
|a Hopkins, Jesse B
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Badeau, Ryan
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Mulichak, Anne M
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Keefe, Lisa J
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Thorne, Robert E
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 20(2013), Pt 1 vom: 01. Jan., Seite 7-13
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
|
| 773 |
1 |
8 |
|g volume:20
|g year:2013
|g number:Pt 1
|g day:01
|g month:01
|g pages:7-13
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1107/S0909049512048303
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_40
|
| 912 |
|
|
|a GBV_ILN_350
|
| 912 |
|
|
|a GBV_ILN_2005
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 20
|j 2013
|e Pt 1
|b 01
|c 01
|h 7-13
|