Directed evolution of nucleotide-based libraries using lambda exonuclease
Directed evolution of nucleotide libraries using recombination or mutagenesis is an important technique for customizing catalytic or biophysical traits of proteins. Conventional directed evolution methods, however, suffer from cumbersome digestion and ligation steps. Here, we describe a simple metho...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1991. - 53(2012), 6 vom: 10. Dez., Seite 357-64
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| 1. Verfasser: |
Lim, Bee Nar
(VerfasserIn) |
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| Weitere Verfasser: |
Choong, Yee Siew,
Ismail, Asma,
Glökler, Jörn,
Konthur, Zoltán,
Lim, Theam Soon |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Fluorescent Dyes
Viral Proteins
DNA Polymerase I
EC 2.7.7.7
Exonucleases
EC 3.1.- |
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