The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning

The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning

© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1990. - 197(2013), 2 vom: 27. Jan., Seite 468-480
1. Verfasser: Shi, Jian Xin (VerfasserIn)
Weitere Verfasser: Adato, Avital, Alkan, Noam, He, Yonghua, Lashbrooke, Justin, Matas, Antonio J, Meir, Sagit, Malitsky, Sergey, Isaacson, Tal, Prusky, Dov, Leshkowitz, Dena, Schreiber, Lukas, Granell, Antonio R, Widemann, Emilie, Grausem, Bernard, Pinot, Franck, Rose, Jocelyn K C, Rogachev, Ilana, Rothan, Christophe, Aharoni, Asaph
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2013
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Membrane Lipids Plant Proteins Recombinant Proteins Transcription Factors Waxes cutin 54990-88-4
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245 1 4 |a The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning 
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520 |a Fleshy tomato fruit typically lacks stomata; therefore, a proper cuticle is particularly vital for fruit development and interaction with the surroundings. Here, we characterized the tomato SlSHINE3 (SlSHN3) transcription factor to extend our limited knowledge regarding the regulation of cuticle formation in fleshy fruits. We created SlSHN3 overexpressing and silenced plants, and used them for detailed analysis of cuticular lipid compositions, phenotypic characterization, and the study on the mode of SlSHN3 action. Heterologous expression of SlSHN3 in Arabidopsis phenocopied overexpression of the Arabidopsis SHNs. Silencing of SlSHN3 results in profound morphological alterations of the fruit epidermis and significant reduction in cuticular lipids. We demonstrated that SlSHN3 activity is mediated by control of genes associated with cutin metabolism and epidermal cell patterning. As with SlSHN3 RNAi lines, mutation in the SlSHN3 target gene, SlCYP86A69, resulted in severe cutin deficiency and altered fruit surface architecture. In vitro activity assays demonstrated that SlCYP86A69 possesses NADPH-dependent ω-hydroxylation activity, particularly of C18:1 fatty acid to the 18-hydroxyoleic acid cutin monomer. This study provided insights into transcriptional mechanisms mediating fleshy fruit cuticle formation and highlighted the link between cutin metabolism and the process of fruit epidermal cell patterning 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Research Support, U.S. Gov't, Non-P.H.S. 
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650 7 |a Plant Proteins  |2 NLM 
650 7 |a Recombinant Proteins  |2 NLM 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Waxes  |2 NLM 
650 7 |a cutin  |2 NLM 
650 7 |a 54990-88-4  |2 NLM 
700 1 |a Adato, Avital  |e verfasserin  |4 aut 
700 1 |a Alkan, Noam  |e verfasserin  |4 aut 
700 1 |a He, Yonghua  |e verfasserin  |4 aut 
700 1 |a Lashbrooke, Justin  |e verfasserin  |4 aut 
700 1 |a Matas, Antonio J  |e verfasserin  |4 aut 
700 1 |a Meir, Sagit  |e verfasserin  |4 aut 
700 1 |a Malitsky, Sergey  |e verfasserin  |4 aut 
700 1 |a Isaacson, Tal  |e verfasserin  |4 aut 
700 1 |a Prusky, Dov  |e verfasserin  |4 aut 
700 1 |a Leshkowitz, Dena  |e verfasserin  |4 aut 
700 1 |a Schreiber, Lukas  |e verfasserin  |4 aut 
700 1 |a Granell, Antonio R  |e verfasserin  |4 aut 
700 1 |a Widemann, Emilie  |e verfasserin  |4 aut 
700 1 |a Grausem, Bernard  |e verfasserin  |4 aut 
700 1 |a Pinot, Franck  |e verfasserin  |4 aut 
700 1 |a Rose, Jocelyn K C  |e verfasserin  |4 aut 
700 1 |a Rogachev, Ilana  |e verfasserin  |4 aut 
700 1 |a Rothan, Christophe  |e verfasserin  |4 aut 
700 1 |a Aharoni, Asaph  |e verfasserin  |4 aut 
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