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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1111/vcp.12004
|2 doi
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|a pubmed24n0743.xml
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|a (DE-627)NLM223121495
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|a (NLM)23205885
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a O'Neil, Elizabeth
|e verfasserin
|4 aut
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|a Comparison of white and red blood cell estimates in urine sediment with hemocytometer and automated counts in dogs and cats
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 28.08.2013
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|a Date Revised 20.10.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 American Society for Veterinary Clinical Pathology.
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|a BACKGROUND: Therapeutic decisions regarding urinalysis are commonly based on the presence of white and red blood cells. Traditionally, numbers per high-power field are estimated using wet-mount microscopic examination. This technique is not standardized and counts are likely prone to inaccuracy. In addition, differentiation of leukocyte types is not possible
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|a OBJECTIVES: The aims of this study were to (1) compare WBC and RBC estimates using wet-mount examination with counts obtained using a hemocytometer, (2) assess if a hematology automated analyzer (Sysmex ST-2000iV/XT) provides reliable WBC and RBC counts in urine comparable to hemocytometer counts, and (3) evaluate air-dried Wright-Giemsa-stained urine drop sediment preparations for the determination of differential leukocyte counts
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|a METHODS: WBC and RBC counts were obtained by performing wet-mount estimates, manual hemocytometer counts, and Sysmex automated counts on 219 canine and feline urine samples. Results were correlated using Spearman rank correlation. Air-dried Wright-Giemsa stained sediment drop preparations (n = 215) were examined for differential counts of leukocytes
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|a RESULTS: A low but significant association was found between WBC estimates on wet-mount examination and hemocytometer counts (rho = 0.37, P < .01). There was a high and significant association when RBC counts were compared between wet-mount and hemocytometer evaluation (rho = 0.7, P < .01). There was very high and significant interassay correlation between Sysmex data from duplicate samples for what the analyzer classified as WBC (rho = 0.97, P < .01) and RBC (rho = 0.94, P < .01). Low correlations were found between the Sysmex RBC counts and both wet-mount estimates and hemocytometer RBC counts (rho = 0.43, P < .01 and rho = 0.39, P < .01, respectively). Cell preservation in the air-dried sediment preparations was so poor that differential counts could not be performed
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|a CONCLUSION: WBC and RBC estimates on wet-mount examination agreed with hemocytometer counts and are therefore considered adequate. The Sysmex ST-2000iV/XT did not provide reliable cell counts under the conditions used
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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1 |
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|a Burton, Shelley
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Horney, Barbara
|e verfasserin
|4 aut
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| 700 |
1 |
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|a MacKenzie, Allan
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Veterinary clinical pathology
|d 1975
|g 42(2013), 1 vom: 21. März, Seite 78-84
|w (DE-627)NLM098159984
|x 1939-165X
|7 nnns
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| 773 |
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|g volume:42
|g year:2013
|g number:1
|g day:21
|g month:03
|g pages:78-84
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|u http://dx.doi.org/10.1111/vcp.12004
|3 Volltext
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|h 78-84
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