Nicotine production by tissue cultures of tobacco as influenced by various culture parameters
Copyright © 1985 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved.
| Veröffentlicht in: | Journal of plant physiology. - 1979. - 118(1985), 5 vom: 08. Apr., Seite 463-70 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1985
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| Zugriff auf das übergeordnete Werk: | Journal of plant physiology |
| Schlagworte: | Journal Article |
| Zusammenfassung: | Copyright © 1985 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved. Tissue culture systems of tobacco (Nicotiana tabacum L., Nicotiana rustica L.) are described, in which up to 5% and more nicotine accumulated on a dry weight basis. Maximum amounts of nicotine (5.3 %, yield 920 mg/1) were detected in mixotrophic green cell suspensions of a newly developed flue-cured tobacco cultivar (N. tabacum cv. Delcrest X cv. McNair 133). This cultivar also showed maximum nicotine content compared to other strains when cultured as callus or heterotrophic cell suspension. Nicotine content was increased up to 29-fold (2. 9 %, yield 360 mg/1) compared to the stock cultures, when a two- or three-stage culture was employed by reducing the auxin and cytokinin levels, raising medium sucrose to 40 gil and replacing ammonium nitrate by potassium nitrate in the production medium. Cells in 20 1 batch fermentor cultures accumulated up to 1 % nicotine with a final yield of 28 mg/1, thus producing much less nicotine |
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| Beschreibung: | Date Completed 03.12.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/S0176-1617(85)80206-6 |