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231224s1984 xx |||||o 00| ||eng c |
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|a 10.1016/S0176-1617(84)80024-3
|2 doi
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|a pubmed25n0743.xml
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|a (PII)S0176-1617(84)80024-3
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|a eng
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|a Rubluo, A
|e verfasserin
|4 aut
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|a Plant Regeneration from Pea Leaflets Cultured in vitro and Genetic Stability of Regenerants
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|c 1984
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 03.12.2012
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved.
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|a Bud and shoot formation and regeneration of complete plants have been achieved with immature and mature leaflets, at various frequency, under the influence of a range of cytokinin and auxin combinations. Immature leaflets, 0.9 to 1.8 mm in length, produced shoots in 26 to 38% explants on a medium containing 10.0 μM BA and NAA, IBA or IAA. A reduction in the frequency of bud and shoot regeneration to 7% in the presence of 10.0 μM each of BA + NAA was observed with mature leaflets. In the differentiation of buds and shoots, no stimulating effect of GA(3) has been observed. Combinations of cytokinins with picloram and 2,4-D suppressed organogenesis. Plant regeneration was influenced by genotype and temperatures. Seed-derived progeny of regenerants contained the normal diploid number of chromosomes (2n = 14). Analysis of four isozymes, esterase, GDH, 6-PGD, and LAP, as well as nine genetically defined morphological characters indicated retention of genetic stability in the progeny of tisssue culture propagules
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|a Journal Article
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|a Kartha, K K
|e verfasserin
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|a Mroginski, L A
|e verfasserin
|4 aut
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|a Dyck, J
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 117(1984), 2 vom: 15. Dez., Seite 119-30
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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|g volume:117
|g year:1984
|g number:2
|g day:15
|g month:12
|g pages:119-30
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|u http://dx.doi.org/10.1016/S0176-1617(84)80024-3
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|d 117
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