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231224s1984 xx |||||o 00| ||eng c |
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|a 10.1016/S0176-1617(84)80125-X
|2 doi
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|a DE-627
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|a eng
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|a Mhatre, M
|e verfasserin
|4 aut
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|a Plant Regeneration in Protoplast Cultures of Tylophora indica
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|c 1984
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 03.12.2012
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|a Date Revised 10.03.2022
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Copyright © 1984 Gustav Fischer Verlag, Stuttgart. Published by Elsevier GmbH.. All rights reserved.
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|a Protoplasts isolated from callus tissue derived from freshly cultured stem segments of Tylophora indica (Asclepiadaceae) rapidly divided and resulted in callus. Subsequent embryoid/ shoot bud differentiation in callus was observed on MS medium supplemented with auxins and cytokinins. The embryoids/shoot buds on transfer to appropriate basal medium without growth substances grew into plantlets. Protoplasts isolated from callus tissue maintained for over five years showed divisions and subsequent callus formation but failed to regenerate plants
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|a Journal Article
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|a Bapat, V A
|e verfasserin
|4 aut
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|a Rao, P S
|e verfasserin
|4 aut
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 115(1984), 3 vom: 29. Juli, Seite 231-5
|w (DE-627)NLM098174622
|x 1618-1328
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|g volume:115
|g year:1984
|g number:3
|g day:29
|g month:07
|g pages:231-5
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|u http://dx.doi.org/10.1016/S0176-1617(84)80125-X
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