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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la303661p
|2 doi
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|a pubmed24n0742.xml
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|a (DE-627)NLM222634456
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|a (NLM)23153411
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Wang, Tuo
|e verfasserin
|4 aut
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| 245 |
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|a Aggregation and dynamics of oligocholate transporters in phospholipid bilayers revealed by solid-state NMR spectroscopy
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 20.05.2013
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Macrocycles made of cholate building blocks were previously found to transport glucose readily across lipid bilayers. In this study, an (15)N, (13)Cα-labeled glycine was inserted into a cyclic cholate trimer and attached at the end of a linear trimer, respectively. The isotopic labeling allowed us to use solid-state NMR spectroscopy to study the dynamics, aggregation, and depth of insertion of these compounds in lipid membranes. The cyclic compound was found to be mostly immobilized in DLPC, POPC/POPG, and POPC/POPG/cholesterol membranes, whereas the linear trimer displayed large-amplitude motion that depended on the membrane thickness and viscosity. (13)C-detected (1)H spin diffusion experiments revealed the depth of insertion of the compounds in the membranes, as well as their contact with water molecules. The data support a consistent stacking model for the cholate macrocycles in lipid membranes, driven by the hydrophobic interactions of the water molecules in the interior of the macrocycles. The study also shows a strong preference of the linear trimer for the membrane surface, consistent with its lack of transport activity in earlier liposome leakage assays
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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|a Carbon Isotopes
|2 NLM
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|a Cholates
|2 NLM
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|a Lipid Bilayers
|2 NLM
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|a Membrane Transport Proteins
|2 NLM
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|a Nitrogen Isotopes
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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|a Phosphatidylglycerols
|2 NLM
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|a Water
|2 NLM
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|a 059QF0KO0R
|2 NLM
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|a 1,2-linoleoylphosphatidylcholine
|2 NLM
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| 650 |
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|a 6542-05-8
|2 NLM
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| 650 |
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|a 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol
|2 NLM
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| 650 |
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|a 81490-05-3
|2 NLM
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| 650 |
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|a Cholesterol
|2 NLM
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|a 97C5T2UQ7J
|2 NLM
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| 650 |
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|a 1-palmitoyl-2-oleoylphosphatidylcholine
|2 NLM
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| 650 |
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|a TE895536Y5
|2 NLM
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| 700 |
1 |
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|a Widanapathirana, Lakmini
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Yan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hong, Mei
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 49 vom: 11. Dez., Seite 17071-8
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
1 |
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|g volume:28
|g year:2012
|g number:49
|g day:11
|g month:12
|g pages:17071-8
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|u http://dx.doi.org/10.1021/la303661p
|3 Volltext
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