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|a 10.1270/jsbbs.61.462
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|a eng
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1 |
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|a Anai, Toyoaki
|e verfasserin
|4 aut
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|a Potential of a mutant-based reverse genetic approach for functional genomics and molecular breeding in soybean
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|c 2012
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Completed 09.11.2012
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|a Date Revised 12.05.2024
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Mutant-based reverse genetics offers a powerful way to create novel mutant alleles at a selected locus. This approach makes it possible to directly identify plants that carry a specific modified gene from the nucleotide sequence data. Soybean [Glycine max (L.) Merr.] has a highly redundant paleopolyploid genome (approx. 1.1 Gb), which was completely sequenced in 2010. Using reverse genetics to support functional genomics studies designed to predict gene function would accelerate post-genomics research in soybean. Furthermore, the novel mutant alleles created by this approach would be useful genetic resources for improving various traits in soybean. A reverse genetic screening platform in soybean has been developed that combines more than 40,000 mutant lines with a high-throughput method, Targeting Local Lesions IN Genome (TILLING). In this review, the mutant-based reverse genetic approach based on this platform is described, and the likely evolution of this approach in the near future
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|a Journal Article
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| 650 |
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|a TILLING
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| 650 |
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|a mutagenesis
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| 650 |
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4 |
|a reverse genetics
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| 650 |
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|a soybean
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| 773 |
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|i Enthalten in
|t Breeding science
|d 1998
|g 61(2012), 5 vom: 18. Jan., Seite 462-7
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|x 1344-7610
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|g year:2012
|g number:5
|g day:18
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|g pages:462-7
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|u http://dx.doi.org/10.1270/jsbbs.61.462
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