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231224s2012 xx |||||o 00| ||eng c |
| 024 |
7 |
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|a 10.1021/la303987c
|2 doi
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|a pubmed24n0741.xml
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|a (DE-627)NLM222451920
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|a (NLM)23134267
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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1 |
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|a González-Campo, Arántzazu
|e verfasserin
|4 aut
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|a Supramolecularly oriented immobilization of proteins using cucurbit[8]uril
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 23.07.2013
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|a Date Revised 25.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a A supramolecular strategy is used for oriented positioning of proteins on surfaces. A viologen-based guest molecule is attached to the surface, while a naphthol guest moiety is chemoselectively ligated to a yellow fluorescent protein. Cucurbit[8]uril (CB[8]) is used to link the proteins onto surfaces through specific charge-transfer interactions between naphthol and viologen inside the CB cavity. The assembly process is characterized using fluorescence and atomic force microscopy, surface plasmon resonance, IR-reflective absorption, and X-ray photoelectron spectroscopy measurements. Two different immobilization routes are followed to form patterns of the protein ternary complexes on the surfaces. Each immobilization route consists of three steps: (i) attaching the viologen to the glass using microcontact chemistry, (ii) blocking, and (iii) either incubation or microcontact printing of CB[8] and naphthol guests. In both cases uniform and stable fluorescent patterns are fabricated with a high signal-to-noise ratio. Control experiments confirm that CB[8] serves as a selective linking unit to form stable and homogeneous ternary surface-bound complexes as envisioned. The attachment of the yellow fluorescent protein complexes is shown to be reversible and reusable for assembly as studied using fluorescence microscopy
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Bridged-Ring Compounds
|2 NLM
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|a Imidazoles
|2 NLM
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|a Immobilized Proteins
|2 NLM
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|a Luminescent Proteins
|2 NLM
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|a Naphthols
|2 NLM
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| 650 |
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|a Quaternary Ammonium Compounds
|2 NLM
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| 650 |
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|a cucurbit(8)uril
|2 NLM
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|a Brasch, Melanie
|e verfasserin
|4 aut
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|a Uhlenheuer, Dana A
|e verfasserin
|4 aut
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|a Gómez-Casado, Alberto
|e verfasserin
|4 aut
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| 700 |
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|a Yang, Lanti
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Brunsveld, Luc
|e verfasserin
|4 aut
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| 700 |
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|a Huskens, Jurriaan
|e verfasserin
|4 aut
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|a Jonkheijm, Pascal
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 47 vom: 27. Nov., Seite 16364-71
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:28
|g year:2012
|g number:47
|g day:27
|g month:11
|g pages:16364-71
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|u http://dx.doi.org/10.1021/la303987c
|3 Volltext
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