Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion

Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion

Copyright © 2012 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 145(2012), 3 vom: 14. Dez., Seite 189-200
1. Verfasser: Berthet, Julien (VerfasserIn)
Weitere Verfasser: Damien, Pauline, Hamzeh-Cognasse, Hind, Arthaud, Charles-Antoine, Eyraud, Marie-Ange, Zéni, Fabrice, Pozzetto, Bruno, McNicol, Archibald, Garraud, Olivier, Cognasse, Fabrice
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Comparative Study Journal Article Research Support, Non-U.S. Gov't CD63 protein, human CXCL8 protein, human Cytokines IL6 protein, human Interleukin-6 Interleukin-8 Lipopolysaccharide Receptors mehr... Lipopolysaccharides P-Selectin Protein Isoforms SELP protein, human TLR4 protein, human Tetraspanin 30 Toll-Like Receptor 4 Tumor Necrosis Factor-alpha
Beschreibung
Zusammenfassung:Copyright © 2012 Elsevier Inc. All rights reserved.
Platelets are currently acknowledged as cells of innate immunity and inflammation and play a complex role in sepsis. We examined whether different types of LPS have different effects on the release of soluble signaling/effective molecules from platelets. We used platelet-rich plasma from healthy volunteers and LPS from two strains of gram-negative bacteria with disparate LPS structures. We combined LPS-stimulated platelet supernatants with reporter cells and measured the PBMC cytokine secretion profiles. Upon stimulation of platelets with both Escherichia coli O111 and Salmonella minnesota LPS, the platelet LPS::TLR4 interaction activated pathways to trigger the production of a large number of molecules. The different platelet supernatants caused differential PBMC secretion of IL-6, TNFα, and IL-8. Our data demonstrate that platelets have the capacity to sense external signals differentially through a single type of pathogen recognition receptor and adjust the innate immune response appropriately for pathogens exhibiting different types of 'danger' signals
Beschreibung:Date Completed 17.01.2013
Date Revised 16.08.2019
published: Print-Electronic
CommentIn: Clin Immunol. 2013 Jan;146(1):13-4. - PMID 23168607
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2012.09.004