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231224s2013 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2012.08.021
|2 doi
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|a pubmed24n0740.xml
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|a (PII)S0176-1617(12)00402-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhang, Yanping
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Bioinformatics prediction of miRNAs in the Prunus persica genome with validation of their precise sequences by miR-RACE
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|c 2013
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 10.05.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier GmbH. All rights reserved.
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|a We predicted 262 potential MicroRNAs (miRNAs) belonging to 70 miRNA families from the peach (Prunus persica) genome and two specific 5' and 3' miRNA rapid amplification of cDNA ends (miR-RACE) PCR reactions and sequence-directed cloning were employed to accurately validate 61 unique P. persica miRNAs (Ppe-miRNAs) sequences belonging to 61 families comprising 97 Ppe-miRNAs. Validation of the termini nucleotides in particular can define the real sequences of the Ppe-miRNAs on peach genome. Comparison between predicted and validated Ppe-miRNAs through alignment revealed that 43 unique orthologous sequences were identical, while the remaining 18 exhibited some divergences at their termini nucleotides. Quantitative real-time polymerase chain reaction (qRT-PCR) was further employed to analyze the expression of all the 61 miRNAs and 10 putative targets of 8 randomly selected Ppe-miRNAs in peach leaves, flowers and fruits at different stages of development, where both the miRNAs and the putative target genes showed tissue-specific expression
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a DNA, Complementary
|2 NLM
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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|a RNA, Plant
|2 NLM
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| 700 |
1 |
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|a Bai, Youhuang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Han, Jian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kayesh, Emrul
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Weibing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Fang, Jinggui
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 170(2013), 1 vom: 01. Jan., Seite 80-92
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
1 |
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|g volume:170
|g year:2013
|g number:1
|g day:01
|g month:01
|g pages:80-92
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|u http://dx.doi.org/10.1016/j.jplph.2012.08.021
|3 Volltext
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