The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy

Copyright © 2012 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 145(2012), 2 vom: 05. Nov., Seite 161-73
1. Verfasser: He, Yin-Yan (VerfasserIn)
Weitere Verfasser: He, Xiao-Ju, Guo, Pei-Fen, Du, Mei-Rong, Shao, Jun, Li, Ming-Qing, Li, Da-Jin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antigens, CD CCL2 protein, human CCR2 protein, human Chemokine CCL2 Culture Media, Conditioned GATA3 Transcription Factor Receptors, CCR2 Recombinant Proteins
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245 1 4 |a The decidual stromal cells-secreted CCL2 induces and maintains decidual leukocytes into Th2 bias in human early pregnancy 
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520 |a The precise mechanism of characteristic Th2 predominance at maternal-fetal interface remains unresolved. In the present study, we investigated roles of the decidua-derived CCL2 in Th2 predominance at maternal-fetal interface. FCM shows that 55% CD56(+)CD16(-)CD3(-) decidual NK, 52% CD4(+) T cells and 75% CD14(+) monocytes express CCR2. Recombinant human CCL2 (rhCCL2) and the decidual stromal cells (DSCs)-derived supernatant can enhance proliferation and inhibit apoptosis of these decidual leukocytes (DLCs), and promote Th2 cytokines production, IL-4 and IL-10, with an increase in GATA-3 transcription. They also inhibit the secretion of Th1 cytokines, TNF-α and IFN-γ, with a decrease in T-bet transcription It is concluded that the secreted CCL2 by decidual stromal cells increases GATA-3 transcription and decreases T-bet transcription in the decidual leukocytes, which contributes to Th2 polarization at maternal-fetal interface. Furthermore, the Th2 cytokines, IL-4 and IL-10, rather than Th1 cytokines, was shown to increase CCL2 secretion of DSC 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Antigens, CD  |2 NLM 
650 7 |a CCL2 protein, human  |2 NLM 
650 7 |a CCR2 protein, human  |2 NLM 
650 7 |a Chemokine CCL2  |2 NLM 
650 7 |a Culture Media, Conditioned  |2 NLM 
650 7 |a GATA3 Transcription Factor  |2 NLM 
650 7 |a Receptors, CCR2  |2 NLM 
650 7 |a Recombinant Proteins  |2 NLM 
700 1 |a He, Xiao-Ju  |e verfasserin  |4 aut 
700 1 |a Guo, Pei-Fen  |e verfasserin  |4 aut 
700 1 |a Du, Mei-Rong  |e verfasserin  |4 aut 
700 1 |a Shao, Jun  |e verfasserin  |4 aut 
700 1 |a Li, Ming-Qing  |e verfasserin  |4 aut 
700 1 |a Li, Da-Jin  |e verfasserin  |4 aut 
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