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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2012.04350.x
|2 doi
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|a pubmed25n0739.xml
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|a (DE-627)NLM22169241X
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|a (NLM)23050791
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Nishizawa-Yokoi, Ayako
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 25.04.2013
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| 500 |
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.
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|a Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in Agrobacterium-mediated transferred DNA (T-DNA) integration into the genome of the model plant Arabidopsis remains inconclusive. Having established a rapid and highly efficient Agrobacterium-mediated transformation system in rice (Oryza sativa) using scutellum-derived calli, we examined here the involvement of the NHEJ pathway in Agrobacterium-mediated stable transformation in rice. Rice calli from OsKu70, OsKu80 and OsLig4 knockdown (KD) plants were infected with Agrobacterium harboring a sensitive emerald luciferase (LUC) reporter construct to evaluate stable expression and a green fluorescent protein (GFP) construct to monitor transient expression of T-DNA. Transient expression was not suppressed, but stable expression was reduced significantly, in KD plants. Furthermore, KD-Ku70 and KD-Lig4 calli exhibited an increase in the frequency of homologous recombination (HR) compared with control calli. In addition, suppression of OsKu70, OsKu80 and OsLig4 induced the expression of HR-related genes on treatment with DNA-damaging agents. Our findings suggest strongly that NHEJ is involved in Agrobacterium-mediated stable transformation in rice, and that there is a competitive and complementary relationship between the NHEJ and HR pathways for DNA double-strand break repair in rice
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a DNA, Bacterial
|2 NLM
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|a DNA-Binding Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a T-DNA
|2 NLM
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|a Green Fluorescent Proteins
|2 NLM
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|a 147336-22-9
|2 NLM
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|a DNA Helicases
|2 NLM
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| 650 |
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|a EC 3.6.4.-
|2 NLM
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| 650 |
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|a DNA Ligases
|2 NLM
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| 650 |
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|a EC 6.5.1.-
|2 NLM
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| 650 |
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|a DNA Ligase ATP
|2 NLM
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| 650 |
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|a EC 6.5.1.1
|2 NLM
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| 700 |
1 |
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|a Nonaka, Satoko
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Saika, Hiroaki
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kwon, Yong-Ik
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Osakabe, Keishi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Toki, Seiichi
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t The New phytologist
|d 1990
|g 196(2012), 4 vom: 10. Dez., Seite 1048-1059
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
1 |
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|g volume:196
|g year:2012
|g number:4
|g day:10
|g month:12
|g pages:1048-1059
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| 856 |
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|u http://dx.doi.org/10.1111/j.1469-8137.2012.04350.x
|3 Volltext
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|d 196
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|e 4
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|h 1048-1059
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