New gene construction strategy in T-DNA vector to enhance expression level of sweet potato sporamin and insect resistance in transgenic Brassica oleracea
Copyright © 2006 Elsevier Ireland Ltd. All rights reserved.
| Veröffentlicht in: | Plant science : an international journal of experimental plant biology. - 1985. - 171(2006), 3 vom: 01. Sept., Seite 367-74 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Zugriff auf das übergeordnete Werk: | Plant science : an international journal of experimental plant biology |
| Schlagworte: | Journal Article |
| Zusammenfassung: | Copyright © 2006 Elsevier Ireland Ltd. All rights reserved. Sporamin, an abundant storage protein in tuberous roots of sweet potato, possesses strong inhibitory activity against trypsin and pest-resistance. To promote consistent high-level expression of sporamin and insect resistance in transgenic Brassica plants, a wound-responsive sporamin promoter (Pspoa) alone or combined with matrix-attached-region-like DNA segment (spoMAR) were constructed for driving sporamin cDNA. The results showed the transgenic plants containing Pspoa-drived sporamin and spoMAR displayed the highest level and low inter-transformant variability of sporamin expression, and the ability of insect resistance of transformants positively correlated with sporamin activity. Furthermore, expressions of Pspoa-drived sporamin especially combined with the spoaMAR retains high and steady levels in the T(1) and T(2) generations, in marked contrast to the variable expression patterns observed in CaMV35S promoter-driven transformants. This study evidently indicates that the Pspoa and spoaMAR would be very efficient for high transgene expression in plants and obtaining inherently stable transformants in consecutive progenies |
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| Beschreibung: | Date Completed 02.10.2012 Date Revised 30.09.2020 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2006.04.003 |