Streamlined extract preparation for Escherichia coli-based cell-free protein synthesis by sonication or bead vortex mixing
Escherichia coli-based cell extract is a vital component of inexpensive and high-yielding cell-free protein synthesis reactions. However, effective preparation of E. coli cell extract is limited to high-pressure (French press-style or impinge-style) or bead mill homogenizers, which all require a sig...
| Veröffentlicht in: | BioTechniques. - 1991. - 53(2012), 3 vom: 10. Sept., Seite 163-74 |
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| Weitere Verfasser: | , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Cell Extracts Escherichia coli Proteins Muramidase EC 3.2.1.17 |
| Online verfügbar |
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