Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins. The system uses (i) a cell lysate that is mildly prepared under slightly reduced conditions, thus maintaining the ac...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 53(2012), 3 vom: 10. Sept., Seite 153-60
|
|---|
| 1. Verfasser: |
Merk, Helmut
(VerfasserIn) |
|---|
| Weitere Verfasser: |
Gless, Christine,
Maertens, Barbara,
Gerrits, Michael,
Stiege, Wolfgang |
|---|
| Format: | Online-Aufsatz
|
|---|
| Sprache: | English |
|---|
| Veröffentlicht: |
2012
|
|---|
| Zugriff auf das übergeordnete Werk: | BioTechniques
|
|---|
| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Disulfides
Endotoxins
Immunoglobulin Fab Fragments
Recombinant Fusion Proteins
Erythropoietin
11096-26-7
hen egg lysozyme
EC 3.2.1.-
mehr...
Muramidase
EC 3.2.1.17 |
|---|