Validation of Advia plateletcrit for assessing platelet mass in dogs, including Cavalier King Charles spaniels

© 2012 American Society for Veterinary Clinical Pathology.

Détails bibliographiques
Publié dans:Veterinary clinical pathology. - 1975. - 41(2012), 3 vom: 17. Sept., Seite 336-43
Auteur principal: Tvedten, Harold W (Auteur)
Autres auteurs: Lilliehöök, Inger E, Oberg, Josefine, Häggström, Jens, Höglund, Katja, Ljungvall, Ingrid
Format: Article en ligne
Langue:English
Publié: 2012
Accès à la collection:Veterinary clinical pathology
Sujets:Journal Article Validation Study
Description
Résumé:© 2012 American Society for Veterinary Clinical Pathology.
BACKGROUND: Determination of the plateletcrit (PCT) is the most effective way to evaluate platelet mass in dogs, such as Cavalier King Charles spaniel (CKCS) dogs, with macrothrombocytopenia. The IDEXX VetAutoread hematology analyzer, which performs quantitative buffy coat (QBC) analysis, has been validated to determine platelet mass in CKCS dogs. The Advia 2120 reports a PCT, but the validity of this value has not been evaluated for dogs with macrothrombocytopenia
OBJECTIVES: The goal of this study was to validate MPV and PCT determined by the Advia 2120 in dogs, including CKCS dogs, comparing values with those obtained from QBC analysis
METHODS: Advia PCT was compared with QBC results from 43 CKCS dogs and 15 dogs of other breeds in one study. Advia PCT, platelet count, and MPV were evaluated to identify biologic patterns in 31 clinically healthy CKCS dogs and 66 dogs of 3 other breeds and to generate values used for comparisons
RESULTS: Advia PCT agreed well with QBC results in general, but had a negative bias and appeared to underestimate PCT in CKCS dogs with the lowest PCTs. Advia PCT and MPV results followed expected biologic patterns in CKCS dogs and dogs of other breeds with MPVs being highest in dogs with the lowest platelet counts
CONCLUSIONS: Advia 2120 PCT and MPV satisfactorily identified changes in platelet mass and size in CKCS dogs, but PCTs were lower than expected, especially in CKCS dogs with the lowest PCTs, when compared with QBC results
Description:Date Completed 26.08.2014
Date Revised 10.12.2019
published: Print
Citation Status MEDLINE
ISSN:1939-165X
DOI:10.1111/j.1939-165X.2012.00455.x