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DE-627 |
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20231224045821.0 |
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231224s2012 xx ||||| 00| ||chi c |
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|a pubmed24n0735.xml
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|a (DE-627)NLM220594341
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|a (NLM)22931945
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Liu, Wei
|e verfasserin
|4 aut
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| 245 |
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|a Oxidative stress inhibits the protective effects of insulin-like growth factor-1 on cortical neurons of neonatal rat
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 14.03.2013
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|a Date Revised 07.06.2016
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To explore the protective effects of insulin-like growth factor-1(IGF-1) on the survival and apoptosis of cortical neurons of neonatal rat under oxidative stress and its significance
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|a METHOD: Primary cortical neurons from newborn rat were cultured and the oxidative stress model was established. Then cells were randomly divided into IGF-1 group and control group. The concentration of LDH in supernatant was detected. Cell survival was determined with MTT assay and the expression of active Caspase-3 was measured using Western Blotting
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|a RESULT: (1) The values of LDH gradually decreased with the increasing IGF-1 added to the cells [(0.5065 ± 0.0064) to (0.435 ± 0.0065), (P < 0.01)], but when the concentration of IGF-1 reached a certain level (> 25 ng/ml), there were no longer obvious effects on the level of LDH [(0.42 ± 0.012) to (0.418 ± 0.0098), (P > 0.05)]; Western blot showed that the level of active Caspase-3 was significantly decreased after treatment with IGF-1 [(0.662 ± 0.033) to (0.199 ± 0.01), (P < 0.01)]. (2) Compared with control group, without or with low concentration of H2O2 (0 - 40 µM), the values of LDH and the expression of active Caspase-3 in IGF-1 group were significantly decreased[(1.518 ± 0.137) to (1.068 ± 0.067), (P < 0.05) and 0.850 ± 0.042 to 0.597 ± 0.03, P < 0.01, respectively] while the values of MTT obviously elevated [(0.773 ± 0.062) to (1.196 ± 0.057), (P < 0.05)]; but with higher concentration (≥ 60 µM) of H2O2, the values of LDH and MTT and the expression of active Caspase-3 in IGF-1 group all had no significant difference (P > 0.05). (3) When the concentration of H2O2 reached 60 µM and higher, whatever concentration of IGF-1 could not lower the level of LDH compared with control group [(2.376 ± 0.04) to (2.442 ± 0.046), (P > 0.05)]
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|a CONCLUSIONS: Oxidative stress can induce IGF-1 resistance of cortical neurons in neonatal rat, and even increasing the concentration of IGF-1 can not restore their sensitivity to IGF-1
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|a English Abstract
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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| 650 |
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|a Neuroprotective Agents
|2 NLM
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| 650 |
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|a Insulin-Like Growth Factor I
|2 NLM
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| 650 |
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|a 67763-96-6
|2 NLM
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|a Hydrogen Peroxide
|2 NLM
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|a BBX060AN9V
|2 NLM
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|a L-Lactate Dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.27
|2 NLM
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| 650 |
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|a Casp3 protein, rat
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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|a Caspase 3
|2 NLM
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| 650 |
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|a EC 3.4.22.-
|2 NLM
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| 700 |
1 |
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|a Chang, Li-wen
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Wen-bin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Zhi-jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Wei-hua
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Zhonghua er ke za zhi = Chinese journal of pediatrics
|d 1960
|g 50(2012), 6 vom: 29. Juni, Seite 455-9
|w (DE-627)NLM136249191
|x 0578-1310
|7 nnns
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| 773 |
1 |
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|g volume:50
|g year:2012
|g number:6
|g day:29
|g month:06
|g pages:455-9
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|d 50
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|h 455-9
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