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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la3011025
|2 doi
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|a pubmed25n0735.xml
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|a (DE-627)NLM220524475
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|a (NLM)22924640
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|a DE-627
|b ger
|c DE-627
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|a eng
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|a Harper-Leatherman, Amanda S
|e verfasserin
|4 aut
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|a Simplified procedure for encapsulating cytochrome c in silica aerogel nanoarchitectures while retaining gas-phase bioactivity
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 06.03.2013
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|a Date Revised 21.11.2013
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Cytochrome c (cyt. c) has been encapsulated in silica sol-gels and processed to form bioaerogels with gas-phase activity for nitric oxide through a simplified synthetic procedure. Previous reports demonstrated a need to adsorb cyt. c to metal nanoparticles prior to silica sol-gel encapsulation and processing to form aerogels. We report that cyt. c can be encapsulated in aerogels without added nanoparticles and retain structural stability and gas-phase activity for nitric oxide. While the UV-visible Soret absorbance and nitric oxide response indicate that cyt. c encapsulated with nanoparticles in aerogels remains slightly more stable and functional than cyt. c encapsulated alone, these properties are not very different in the two types of aerogels. From UV-visible and Soret circular dichroism results, we infer that cyt. c encapsulated alone self-organizes to reduce contact with the silica gel in a way that may bear at least some resemblance to the way cyt. c self-organizes into superstructures of protein within aerogels when nanoparticles are present. Both the buffer concentration and the cyt. c concentration of solutions used to synthesize the bioaerogels affect the structural integrity of the protein encapsulated alone within the dried aerogels. Optimized bioaerogels are formed when cyt. c is encapsulated from 40 mM phosphate buffered solutions, and when the loaded cyt. c concentration in the aerogel is in the range of 5 to 15 μM. Increased viability of cyt. c in aerogels is also observed when supercritical fluid used to produce aerogels is vented over relatively long times
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Gases
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|a Gels
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|a Nitric Oxide
|2 NLM
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|a 31C4KY9ESH
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|a Gold
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|a 7440-57-5
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|a Silicon Dioxide
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|a 7631-86-9
|2 NLM
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|a Cytochromes c
|2 NLM
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|a 9007-43-6
|2 NLM
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|a Iftikhar, Mariam
|e verfasserin
|4 aut
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|a Ndoi, Adela
|e verfasserin
|4 aut
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|a Scappaticci, Steven J
|e verfasserin
|4 aut
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|a Lisi, George P
|e verfasserin
|4 aut
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|a Buzard, Kaitlyn L
|e verfasserin
|4 aut
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|a Garvey, Elizabeth M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 28(2012), 41 vom: 16. Okt., Seite 14756-65
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:28
|g year:2012
|g number:41
|g day:16
|g month:10
|g pages:14756-65
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|u http://dx.doi.org/10.1021/la3011025
|3 Volltext
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