The tomato CAROTENOID CLEAVAGE DIOXYGENASE8 (SlCCD8) regulates rhizosphere signaling, plant architecture and affects reproductive development through strigolactone biosynthesis

© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 196(2012), 2 vom: 12. Okt., Seite 535-547
1. Verfasser: Kohlen, Wouter (VerfasserIn)
Weitere Verfasser: Charnikhova, Tatsiana, Lammers, Michiel, Pollina, Tobia, Tóth, Peter, Haider, Imran, Pozo, María J, de Maagd, Ruud A, Ruyter-Spira, Carolien, Bouwmeester, Harro J, López-Ráez, Juan A
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Lactones Plant Exudates Plant Proteins RNA, Messenger
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100 1 |a Kohlen, Wouter  |e verfasserin  |4 aut 
245 1 4 |a The tomato CAROTENOID CLEAVAGE DIOXYGENASE8 (SlCCD8) regulates rhizosphere signaling, plant architecture and affects reproductive development through strigolactone biosynthesis 
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520 |a Strigolactones are plant hormones that regulate both above- and belowground plant architecture. Strigolactones were initially identified as rhizosphere signaling molecules. In the present work, the tomato (Solanum lycopersicum) CAROTENOID CLEAVAGE DIOXYGENASE 8 (SlCCD8) was cloned and its role in rhizosphere signaling and plant physiology assessed by generating knock-down lines. Transgenic SlCCD8 plants were generated by RNAi-mediated silencing. Lines with different levels of strigolactone reduction--confirmed by UPLC-MS/MS--were selected and their phenotypes investigated. Lines exhibiting reduced SlCCD8 levels displayed increased shoot branching, reduced plant height, increased number of nodes and excessive adventitious root development. In addition, these lines exhibited reproductive phenotypes such as smaller flowers, fruits, as well as fewer and smaller seeds per fruit. Furthermore, we show that strigolactone loading to the xylem sap is possibly restricted to orobanchol. Infestation by Phelipanche ramosa was reduced by 90% in lines with a relatively mild reduction in strigolactone biosynthesis and secretion while arbuscular mycorrhizal symbiosis, apical dominance and fruit yield were only mildly affected. This demonstrates that reduction of strigolactone biosynthesis could be a suitable tool in parasitic weed management. Furthermore, our results suggest that strigolactones are involved in even more physiological processes than so far assumed 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 7 |a Lactones  |2 NLM 
650 7 |a Plant Exudates  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a RNA, Messenger  |2 NLM 
700 1 |a Charnikhova, Tatsiana  |e verfasserin  |4 aut 
700 1 |a Lammers, Michiel  |e verfasserin  |4 aut 
700 1 |a Pollina, Tobia  |e verfasserin  |4 aut 
700 1 |a Tóth, Peter  |e verfasserin  |4 aut 
700 1 |a Haider, Imran  |e verfasserin  |4 aut 
700 1 |a Pozo, María J  |e verfasserin  |4 aut 
700 1 |a de Maagd, Ruud A  |e verfasserin  |4 aut 
700 1 |a Ruyter-Spira, Carolien  |e verfasserin  |4 aut 
700 1 |a Bouwmeester, Harro J  |e verfasserin  |4 aut 
700 1 |a López-Ráez, Juan A  |e verfasserin  |4 aut 
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