Dynamic proteomic profile of potato tuber during its in vitro development

Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant science : an international journal of experimental plant biology. - 1985. - 195(2012) vom: 24. Okt., Seite 1-9
1. Verfasser: Yu, Jae Woong (VerfasserIn)
Weitere Verfasser: Choi, Jong-Soon, Upadhyaya, Chandrama Prakash, Kwon, Sang Oh, Gururani, Mayank Anand, Nookaraju, Akula, Nam, Ju-Hyun, Choi, Chi-Won, Kim, Seung Il, Ajappala, Hemavathi, Kim, Hyun Soon, Jeon, Jae Heung, Park, Se Won
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Plant science : an international journal of experimental plant biology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Plant Proteins Proteome
Beschreibung
Zusammenfassung:Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Potato tuberization is a complicated biochemical process, which is dependent on external environmental factors. Tuber development in potato consists of a series of biochemical and morphological processes at the stolon tip. Signal transduction proteins are involved in the source-sink transition during potato tuberization. In the present study, we examined protein profiles under in vitro tuber-inducing conditions using a shotgun proteomic approach involving denaturing gel electrophoresis and liquid chromatography-mass spectrometry. A total of 251 proteins were identified and classified into 9 groups according to distinctive expression patterns during the tuberization stage. Stolon stage-specific proteins were primarily involved in the photosynthetic machinery. Proteins specific to the initial tuber stage included patatin. Proteins specific to the developing tuber stage included 6-fructokinase, phytoalexin-deficient 4-1, metallothionein II-like protein, and malate dehydrogenase. Novel stage-specific proteins identified during in vitro tuberization were ferredoxin-NADP reductase, 34 kDa porin, aquaporin, calmodulin, ripening-regulated protein, and starch synthase. Superoxide dismutase, dehydroascorbate reductase, and catalase I were most abundantly expressed in the stolon; however, the enzyme activities of these proteins were most activated at the initial tuber. The present shotgun proteomic study provides insights into the proteins that show altered expression during in vitro potato tuberization
Beschreibung:Date Completed 02.01.2013
Date Revised 30.09.2020
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2259
DOI:10.1016/j.plantsci.2012.06.007