Peripheral accumulation of newly produced T and B lymphocytes in natalizumab-treated multiple sclerosis patients

Copyright © 2012 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 145(2012), 1 vom: 04. Okt., Seite 19-26
1. Verfasser: Zanotti, Cinzia (VerfasserIn)
Weitere Verfasser: Chiarini, Marco, Serana, Federico, Sottini, Alessandra, Garrafa, Emirena, Torri, Fabio, Caimi, Luigi, Rasia, Sarah, Capra, Ruggero, Imberti, Luisa
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2012
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Antibodies, Monoclonal, Humanized Natalizumab Protein Isoforms Receptors, Antigen, T-Cell
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520 |a The anti-α4 monoclonal antibody natalizumab inhibits lymphocyte extravasation into the central nervous system and increases peripheral T and B lymphocytes in multiple sclerosis patients. To investigate whether the lymphocyte accumulation was due to a higher lymphocyte production, an altered homeostasis, or a differential transmigration of lymphocyte subsets through endothelia, T-cell receptor excision circles and kappa-deleting recombination excision circles were quantified before and after treatment, T-cell receptor repertoire was analyzed by spectratyping, and T- and B-lymphocyte subset migration was studied using transwell coated with vascular and lymphatic endothelial cells. We found that the number of newly produced T and B lymphocytes is increased because of a high release and of a low propensity of naïve subsets to migrate across endothelial cells. In some patients this resulted in an enlargement of T-cell heterogeneity. Because new lymphocyte production ensures the integrity of immune surveillance, its quantification could be used to monitor natalizumab therapy safety 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
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650 7 |a Receptors, Antigen, T-Cell  |2 NLM 
700 1 |a Chiarini, Marco  |e verfasserin  |4 aut 
700 1 |a Serana, Federico  |e verfasserin  |4 aut 
700 1 |a Sottini, Alessandra  |e verfasserin  |4 aut 
700 1 |a Garrafa, Emirena  |e verfasserin  |4 aut 
700 1 |a Torri, Fabio  |e verfasserin  |4 aut 
700 1 |a Caimi, Luigi  |e verfasserin  |4 aut 
700 1 |a Rasia, Sarah  |e verfasserin  |4 aut 
700 1 |a Capra, Ruggero  |e verfasserin  |4 aut 
700 1 |a Imberti, Luisa  |e verfasserin  |4 aut 
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