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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1007/s10646-012-0976-2
|2 doi
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|a pubmed24n0733.xml
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|a (DE-627)NLM220019886
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|a (NLM)22868904
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Han, Peng
|e verfasserin
|4 aut
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| 245 |
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|a Does transgenic Cry1Ac + CpTI cotton pollen affect hypopharyngeal gland development and midgut proteolytic enzyme activity in the honey bee Apis mellifera L. (Hymenoptera, Apidae)?
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 17.03.2013
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|a Date Revised 21.10.2021
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a The transgenic Cry1Ac (Bt toxin) + CpTI (Cowpea Trypsin Inhibitor) cotton cultivar CCRI41 is increasingly used in China and potential side effects on the honey bee Apis mellifera L. have been documented recently. Two studies have assessed potential lethal and sublethal effects in young bees fed with CCRI41 cotton pollen but no effect was observed on learning capacities, although lower feeding activity in exposed honey bees was noted (antifeedant effect). The present study aimed at providing further insights into potential side effects of CCRI41 cotton on honey bees. Emerging honey bees were exposed to different pollen diets using no-choice feeding protocols (chronic exposure) in controlled laboratory conditions and we aimed at documenting potential mechanisms underneath the CCRI41 antifeedant effect previously reported. Activity of midgut proteolytic enzyme of young adult honey bees fed on CCRI41 cotton pollen were not significantly affected, i.e. previously observed antifeedant effect was not linked to disturbed activity of the proteolytic enzymes in bees' midgut. Hypopharyngeal gland development was assessed by quantifying total extractable proteins from the glands. Results suggested that CCRI41 cotton pollen carries no risk to hypopharyngeal gland development of young adult honey bees. In the two bioassays, honey bees exposed to 1 % soybean trypsin inhibitor were used as positive controls for both midgut proteolytic enzymes and hypopharyngeal gland proteins quantification, and bees exposed to 48 ppb (part per billion) (i.e. 48 ng g(-1)) imidacloprid were used as controls for exposure to a sublethal concentration of toxic product. The results show that the previously reported antifeedant effect of CCRI41 cotton pollen on honey bees is not linked to effects on their midgut proteolytic enzymes or on the development of their hypopharyngeal glands. The results of the study are discussed in the framework of risk assessment of transgenic crops on honey bees
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4 |
|a Journal Article
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|a Research Support, Non-U.S. Gov't
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7 |
|a Bacillus thuringiensis Toxins
|2 NLM
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| 650 |
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7 |
|a Bacterial Proteins
|2 NLM
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| 650 |
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7 |
|a Endotoxins
|2 NLM
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| 650 |
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7 |
|a Hemolysin Proteins
|2 NLM
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| 650 |
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7 |
|a Insecticides
|2 NLM
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| 650 |
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7 |
|a Plant Proteins
|2 NLM
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| 650 |
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7 |
|a Trypsin Inhibitors
|2 NLM
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| 650 |
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7 |
|a insecticidal crystal protein, Bacillus Thuringiensis
|2 NLM
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| 650 |
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7 |
|a Peptide Hydrolases
|2 NLM
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| 650 |
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7 |
|a EC 3.4.-
|2 NLM
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| 700 |
1 |
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|a Niu, Chang-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Biondi, Antonio
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Desneux, Nicolas
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Ecotoxicology (London, England)
|d 1992
|g 21(2012), 8 vom: 21. Nov., Seite 2214-21
|w (DE-627)NLM098212214
|x 1573-3017
|7 nnns
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| 773 |
1 |
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|g volume:21
|g year:2012
|g number:8
|g day:21
|g month:11
|g pages:2214-21
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| 856 |
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|u http://dx.doi.org/10.1007/s10646-012-0976-2
|3 Volltext
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|h 2214-21
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