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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1111/j.1469-8137.2012.04243.x
|2 doi
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|a pubmed24n0733.xml
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Li, Yiyuan
|e verfasserin
|4 aut
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|a A tandem segmental duplication (TSD) in green revolution gene Rht-D1b region underlies plant height variation
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 03.01.2013
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|a Date Revised 09.04.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.
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|a • Rht-D1c (Rht10) carried by Chinese wheat (Triticum aestivum) line Aibian 1 is an allele at the Rht-D1 locus. Among the Rht-1 alleles, little is known about Rht-D1c although it determines an extreme dwarf phenotype in wheat. • Here, we cloned and functionally characterized Rht-D1c using a combination of Southern blotting, target region sequencing, gene expression analysis and transgenic experiments. • We found that the Rht-D1c allele was generated through a tandem segmental duplication (TSD) of a > 1 Mb region, resulting in two copies of the Rht-D1b. Two copies of Rht-D1b in the TSD were three-fold more effective in reducing plant height than a single copy, and transformation with a segment containing the tandemly duplicated copy of Rht-D1b resulted in the same level of reduction of plant height as the original copy in Aibian 1. • Our results suggest that changes in gene copy number are one of the important sources of genetic diversity and some of these changes could be directly associated with important traits in crops
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Genetic Markers
|2 NLM
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|a Xiao, Jianhui
|e verfasserin
|4 aut
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1 |
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|a Wu, Jiajie
|e verfasserin
|4 aut
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1 |
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|a Duan, Jialei
|e verfasserin
|4 aut
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1 |
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|a Liu, Yue
|e verfasserin
|4 aut
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1 |
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|a Ye, Xingguo
|e verfasserin
|4 aut
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1 |
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|a Zhang, Xin
|e verfasserin
|4 aut
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1 |
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|a Guo, Xiuping
|e verfasserin
|4 aut
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1 |
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|a Gu, Yongqiang
|e verfasserin
|4 aut
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1 |
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|a Zhang, Lichao
|e verfasserin
|4 aut
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|a Jia, Jizeng
|e verfasserin
|4 aut
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700 |
1 |
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|a Kong, Xiuying
|e verfasserin
|4 aut
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|i Enthalten in
|t The New phytologist
|d 1979
|g 196(2012), 1 vom: 01. Okt., Seite 282-291
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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|g volume:196
|g year:2012
|g number:1
|g day:01
|g month:10
|g pages:282-291
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|u http://dx.doi.org/10.1111/j.1469-8137.2012.04243.x
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