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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2012.07.011
|2 doi
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|a pubmed24n0732.xml
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|a (DE-627)NLM219838305
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|a (NLM)22846333
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Sakamoto, Tomoaki
|e verfasserin
|4 aut
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|a Rice CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of brassinosteroids in vitro
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 08.01.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier Masson SAS. All rights reserved.
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|a Brassinosteroids are biosynthesized from campesterol via several cytochrome P450 (P450)-catalyzed oxidative reactions. We report the biochemical characterization of two brassinosteroid-biosynthetic P450s from rice: CYP90D2 and CYP90D3. A rice dwarf mutant, ebisu dwarf (d2), which contains a defective copy of CYP90D2, is known to be a brassinosteroid-deficient mutant, and CYP90D2 has been considered to act as a C-3 dehydrogenase. However, in vitro biochemical assays using baculovirus/insect cell-produced proteins revealed that both CYP90D2 and CYP90D3 catalyze C-23 hydroxylation of various 22-hydroxylated brassinosteroids, but with markedly different catalytic efficiencies. Both enzymes preferentially convert (22S,24R)-22-hydroxyergost-4-en-3-one, (22S,24R)-22-hydroxy-5α-ergostan-3-one, and 3-epi-6-deoxocathasterone to the corresponding 23-hydroxylated products, but are less active in the conversion of (22S)-22-hydroxycampesterol and 6-deoxocathasterone, in vitro. Consistently, the levels of 23-hydroxylated products of these intermediates, namely, 6-deoxoteasterone, 3-dehydro-6-deoxoteasterone, and 6-deoxotyphasterol were decreased in d2 mutants. These results indicate that CYP90D2 and CYP90D3 can act as brassinosteroid C-23 hydroxylases in rice
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Brassinosteroids
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Cytochrome P-450 Enzyme System
|2 NLM
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|a 9035-51-2
|2 NLM
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|a Steroid Hydroxylases
|2 NLM
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|a EC 1.14.-
|2 NLM
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|a Ohnishi, Toshiyuki
|e verfasserin
|4 aut
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|a Fujioka, Shozo
|e verfasserin
|4 aut
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|a Watanabe, Bunta
|e verfasserin
|4 aut
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|a Mizutani, Masaharu
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 58(2012) vom: 30. Sept., Seite 220-6
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:58
|g year:2012
|g day:30
|g month:09
|g pages:220-6
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|u http://dx.doi.org/10.1016/j.plaphy.2012.07.011
|3 Volltext
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