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01000naa a22002652 4500 |
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DE-627 |
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20231224043748.0 |
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231224s2012 xx ||||| 00| ||eng c |
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|a (NLM)22838078
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Soto, Esteban
|e verfasserin
|4 aut
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| 245 |
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|a Edwardsiella ictaluri as the causative agent of mortality in cultured Nile tilapia
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| 264 |
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ohne Hilfsmittel zu benutzen
|b n
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|a Band
|b nc
|2 rdacarrier
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|a Date Completed 06.09.2012
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|a Date Revised 30.09.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a Edwardsiella ictaluri was consistently isolated from the spleens, livers, and head kidneys of diseased Nile tilapia Oreochromis niloticus from a farm experiencing mortality events in several culture ponds. We describe the first published outbreak of E. ictaluri-induced edwardsiellosis in Nile tilapia. Pure cultures of the isolated bacteria were characterized both biochemically and molecularly. Biochemical analysis was performed using the API-20E and RapID One systems, and antimicrobial susceptibility was determined by the broth microdilution method. Molecular analysis involved sequencing of the 16S rRNA gene, species-specific real-time polymerase chain reaction (PCR), and PCR-mediated genomic fingerprinting (rep-PCR). Pairwise sequence analysis of the 16S rRNA gene identified the case isolates to be a 100% match to E. ictaluri cultured from channel catfish in the southeastern United States. However, rep-PCR analysis identified the case isolates to be genetically different from representative strains isolated from disease outbreaks in cultured channel catfish in Mississippi. Infectivity challenges (intraperitoneal injection and immersion) demonstrated that a representative E. ictaluri strain isolated from tilapia was pathogenic to naive tilapia, reproducing clinical signs and mortality, thereby establishing Koch's postulates
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|a Journal Article
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| 650 |
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7 |
|a Anti-Bacterial Agents
|2 NLM
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1 |
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|a Griffin, Matt
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Arauz, Maziel
|e verfasserin
|4 aut
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| 700 |
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|a Riofrio, Andres
|e verfasserin
|4 aut
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| 700 |
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|a Martinez, Alexis
|e verfasserin
|4 aut
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| 700 |
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|a Cabrejos, Maria Eugenia
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 24(2012), 2 vom: 10. Juni, Seite 81-90
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnns
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| 773 |
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|g volume:24
|g year:2012
|g number:2
|g day:10
|g month:06
|g pages:81-90
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|d 24
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|h 81-90
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