Overexpression of a multiple stress-responsive gene, ZmMPK4, enhances tolerance to low temperature in transgenic tobacco
Copyright © 2012 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 58(2012) vom: 15. Sept., Seite 174-81 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Plant Proteins RNA, Messenger Reactive Oxygen Species Proline 9DLQ4CIU6V Hydrogen Peroxide BBX060AN9V Catalase mehr... |
| Zusammenfassung: | Copyright © 2012 Elsevier Masson SAS. All rights reserved. Mitogen-activated protein kinase (MAPK) cascades play important roles in mediating biotic and abiotic stress responses. In this study, we found that ZmMPK4 protein was predominantly localized in the nucleus. Semi-quantitative RT-PCR analysis revealed that the ZmMPK4 transcription in maize leaves was up-regulated by low temperature, high temperature and exogenous signaling molecules such as hydrogen peroxide, methyl jasmonate and ethephon. Hydrogen peroxide acted as second messenger to mediate 4°C-induced up-regulation of ZmMPK4 mRNA. Transgenic tobacco of overexpressing ZmMPK4 accumulated less reactive oxygen species (ROS), more peroxidase and catalase activities, more proline and soluble sugar contents, and more stress-responsive genes expression, leading to enhancing low temperature stress tolerance compared to the control plants. Taken together, these results strongly suggest that ZmMPK4 positively regulates low temperature stress tolerance in plants |
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| Beschreibung: | Date Completed 08.01.2013 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2012.06.020 |