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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2012.04.015
|2 doi
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|a eng
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|a Bae, Eun-Kyung
|e verfasserin
|4 aut
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|a Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba × Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl
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|c 2012
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 08.01.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier Masson SAS. All rights reserved.
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|a The gene expression profiles of hybrid poplar (Populus alba × Populus tremula var. glandulosa) cells in suspension culture after exposure to salinity (NaCl) induced stress were examined by constructing two suppression subtractive hybridization (SSH) libraries. cDNA from non-treated cells was used as a driver and cDNA samples from cell suspension cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells
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|a Journal Article
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|a Plant Proteins
|2 NLM
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|a Sodium Chloride
|2 NLM
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|a 451W47IQ8X
|2 NLM
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| 700 |
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|a Lee, Hyoshin
|e verfasserin
|4 aut
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|a Lee, Jae-Soon
|e verfasserin
|4 aut
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|a Noh, Eun-Woon
|e verfasserin
|4 aut
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|a Choi, Young-Im
|e verfasserin
|4 aut
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|a Lee, Byung-Hyun
|e verfasserin
|4 aut
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|a Choi, Dong-Woog
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 58(2012) vom: 15. Sept., Seite 151-8
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:58
|g year:2012
|g day:15
|g month:09
|g pages:151-8
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|u http://dx.doi.org/10.1016/j.plaphy.2012.04.015
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