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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2012.05.011
|2 doi
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|a pubmed25n0731.xml
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|a (DE-627)NLM219412596
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|a (NLM)22794921
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|a (PII)S0168-9452(12)00108-2
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Li, Xing
|e verfasserin
|4 aut
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| 245 |
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|a Biochemical characterization and identification of a cinnamyl alcohol dehydrogenase from Artemisia annua
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 30.01.2013
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|a Date Revised 30.09.2020
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|a published: Print-Electronic
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|a GENBANK: EU417964
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|a Citation Status MEDLINE
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|a Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
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|a It is well known in the literature that cinnamyl alcohol dehydrogenase (CAD) reduces hydroxycinnamyl aldehydes, such as coumaryl, coniferyl, and sinapyl aldehydes, to their corresponding alcohols in the presence of NADPH, and these alcohols act as the precursors of lignin biosynthesis. Here, we report the isolation of a cDNA encoding an NADP(+)-dependent CAD, designated as AaCAD, from the cDNA library using glandular secretory trichomes of Artemisia annua as the source of mRNA. A phylogenetic analysis indicated that AaCAD was clustered with AtCAD4 and AtCAD5, which were involved in monolignol biosynthesis from Arabidopsis. Semi-quantitative RT-PCR showed that the AaCAD transcript was abundant mostly in leaf and root, followed by flower, and lowest in stem. Functional and enzymatic assays showed that the recombinant enzyme was able to reversibly reduce a variety of common CADs substrates, namely geranial, cinnamyl aldehyde, sinapyl aldehyde, coniferyl aldehyde, and a sesquiterpenoid artemisinic aldehyde, to geraniol, cinnamyl alcohol, sinapyl alcohol, coniferyl alcohol, and artemisinic alcohol respectively. Besides, considering that AaCAD was identified from the glandular secretory trichomes of A. annua, and that the recombinant enzyme exhibited reductase activity by using artemisinic aldehyde as substrate, some possible role of AaCAD in artemisinin biosynthesis is also discussed
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Aldehydes
|2 NLM
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|a Artemisinins
|2 NLM
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|a Lactones
|2 NLM
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|a Plant Growth Regulators
|2 NLM
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|a Alcohol Oxidoreductases
|2 NLM
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| 650 |
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|a EC 1.1.-
|2 NLM
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|a cinnamyl alcohol dehydrogenase
|2 NLM
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| 650 |
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|a EC 1.1.1.195
|2 NLM
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| 650 |
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|a artemisin
|2 NLM
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| 650 |
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|a Y1R67R7XWU
|2 NLM
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| 700 |
1 |
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|a Ma, Dongming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Jianlin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pu, Gaobin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ji, Yunpeng
|e verfasserin
|4 aut
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| 700 |
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|a Lei, Caiyan
|e verfasserin
|4 aut
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| 700 |
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|a Du, Zhigao
|e verfasserin
|4 aut
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| 700 |
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|a Liu, Benye
|e verfasserin
|4 aut
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| 700 |
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|a Ye, Hechun
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Hong
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 193-194(2012) vom: 28. Sept., Seite 85-95
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnns
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| 773 |
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|g volume:193-194
|g year:2012
|g day:28
|g month:09
|g pages:85-95
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|u http://dx.doi.org/10.1016/j.plantsci.2012.05.011
|3 Volltext
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|a AR
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|d 193-194
|j 2012
|b 28
|c 09
|h 85-95
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