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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la302608g
|2 doi
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|a pubmed24n0731.xml
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|a (DE-627)NLM219366063
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|a (NLM)22788539
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Balbino, Tiago A
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Correlation of the physicochemical and structural properties of pDNA/cationic liposome complexes with their in vitro transfection
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 07.12.2012
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|a Date Revised 25.11.2016
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a In this study, we characterized the conventional physicochemical properties of the complexes formed by plasmid DNA (pDNA) and cationic liposomes (CL) composed of egg phosphatidylcholine (EPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) (50/25/25% molar ratio). We found that these properties are nearly unaffected at the studied ranges when the molar charge ratio (R(±)) between the positive charge from the CL and negative charge from pDNA is not close to the isoneutrality region (R(±) = 1). However, the results from in vitro transfection of HeLa cells showed important differences when R(±) is varied, indicating that the relationships between the physicochemical and biological characteristics were not completely elucidated. To obtain information regarding possible liposome structural modifications, small-angle X-ray scattering (SAXS) experiments were performed as a function of R(±) to obtain correlations between structural, physicochemical, and transfection properties. The SAXS results revealed that pDNA/CL complexes can be described as being composed of single bilayers, double bilayers, and multiple bilayers, depending on the R(±) value. Interestingly, for R(±) = 9, 6, and 3, the system is composed of single and double bilayers, and the fraction of the latter increases with the amount of DNA (or a decreasing R(±)) in the system. This information is used to explain the transfection differences observed at an R(±) = 9 as compared to R(±) = 3 and 6. Close to the isoneutrality region (R(±) = 1.8), there was an excess of pDNA, which induced the formation of a fraction of aggregates with multiple bilayers. These aggregates likely provide additional resistance against the release of pDNA during the transfection phenomenon, reflected as a decrease in the transfection level. The obtained results permitted proper correlation of the physicochemical and structural properties of pDNA/CL complexes with the in vitro transfection of HeLa cells by these complexes, contributing to a better understanding of the gene delivery process
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Cations
|2 NLM
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|a Fatty Acids, Monounsaturated
|2 NLM
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|a Liposomes
|2 NLM
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|a Phosphatidylcholines
|2 NLM
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| 650 |
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|a Phosphatidylethanolamines
|2 NLM
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| 650 |
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|a Quaternary Ammonium Compounds
|2 NLM
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| 650 |
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|a 1,2-dielaidoylphosphatidylethanolamine
|2 NLM
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| 650 |
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|a 76391-83-8
|2 NLM
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| 650 |
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|a DNA
|2 NLM
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|a 9007-49-2
|2 NLM
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|a 1,2-dioleoyloxy-3-(trimethylammonium)propane
|2 NLM
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| 650 |
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|a MR86K0XRQP
|2 NLM
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| 700 |
1 |
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|a Gasperini, Antônio A M
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Oliveira, Cristiano L P
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Azzoni, Adriano R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cavalcanti, Leide P
|e verfasserin
|4 aut
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| 700 |
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|a de La Torre, Lucimara G
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 31 vom: 07. Aug., Seite 11535-45
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:28
|g year:2012
|g number:31
|g day:07
|g month:08
|g pages:11535-45
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|u http://dx.doi.org/10.1021/la302608g
|3 Volltext
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