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231224s2012 xx |||||o 00| ||eng c |
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|a 10.1021/la3020404
|2 doi
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|a pubmed24n0731.xml
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|a (DE-627)NLM219304823
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|a (NLM)22780816
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Tao, Houchao
|e verfasserin
|4 aut
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| 245 |
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|a Synthesis and properties of dodecyl trehaloside detergents for membrane protein studies
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|c 2012
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 20.12.2012
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|a Date Revised 07.12.2022
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Sugar-based detergents, mostly derived from maltose or glucose, prevail in the extraction, solubilization, stabilization, and crystallization of membrane proteins. Inspired by the broad use of trehalose for protecting biological macromolecules and lipid bilayer structures, we synthesized new trehaloside detergents for potential applications in membrane protein research. We devised an efficient synthesis of four dodecyl trehalosides, each with the 12-carbon alkyl chain attached to different hydroxyl groups of trehalose, thus presenting a structurally diverse but related family of detergents. The detergent physical properties, including solubility, hydrophobicity, critical micelle concentration (CMC), and size of micelles, were evaluated and compared with the most popular maltoside analogue, β-D-dodecyl maltoside (DDM), which varied from each other due to distinct molecular geometries and possible polar group interactions in resulting micelles. Crystals of 2-dodecyl trehaloside (2-DDTre) were also obtained in methanol, and the crystal packing revealed multiple H-bonded interactions among adjacent trehalose groups. The few trehaloside detergents were tested for the solubilization and stabilization of the nociceptin/orphanin FQ peptide receptor (ORL1) and MsbA, which belong to the G-protein coupled receptor (GPCR) and ATP-binding cassette transporter families, respectively. Our results demonstrated the utility of trehaloside detergents as membrane protein solubilization reagents with the optimal detergents being protein dependent. Continuing development and investigations of trehaloside detergents are attractive, given their interesting and unique chemical-physical properties and potential interactions with membrane lipids
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|a Journal Article
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|a Research Support, N.I.H., Extramural
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|a Detergents
|2 NLM
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|a Glucosides
|2 NLM
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|a Membrane Proteins
|2 NLM
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| 650 |
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|a Receptors, G-Protein-Coupled
|2 NLM
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| 650 |
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|a Receptors, Opioid
|2 NLM
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| 650 |
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|a Nociceptin Receptor
|2 NLM
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| 700 |
1 |
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|a Fu, Yu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thompson, Aaron
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lee, Sung Chang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mahoney, Nicholas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Stevens, Raymond C
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Qinghai
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 28(2012), 30 vom: 31. Juli, Seite 11173-81
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:28
|g year:2012
|g number:30
|g day:31
|g month:07
|g pages:11173-81
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|u http://dx.doi.org/10.1021/la3020404
|3 Volltext
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