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NLM219058318 |
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DE-627 |
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231224s2012 xx ||||| 00| ||chi c |
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|a pubmed25n0730.xml
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|a (DE-627)NLM219058318
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|a (NLM)22748460
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wang, Xiao-hong
|e verfasserin
|4 aut
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| 245 |
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|a The multiplex polymerase chain reaction for detection of ventilator-associated trachea-bronchitis and pneumonia in patients with common pathogens
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| 264 |
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|c 2012
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 29.07.2013
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|a Date Revised 09.12.2020
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|a published: Print
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|a Citation Status MEDLINE
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|a OBJECTIVE: To explore the clinical utility of multiple polymerase chain reaction (M-PCR) in the rapid detection of the common pathogens in ventilator-associated trachea - bronchitis (VAT) and ventilator-associated pneumonia (VAP)
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| 520 |
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|a METHODS: Sputum samples of 75 patients complicated VAT or VAP in surgical intensive care unit (SICU), were examined by bacterial culture, ordinary PCR, the M-PCR detection. The pathogen detection rates among three methods were compared
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| 520 |
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|a RESULTS: The Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae of the positive detection rates were 50.7%, 45.3%, 30.7%, 41.3% and 58.7% by bacterial culture. By ordinary PCR, the positive detection rates were respectively 88.0%, 89.3%, 78.7%, 85.3% and 93.3%, and by M-PCR, the positive detection rates were respectively 92.1%, 90.7%, 82.7%, 89.3% and 96.0%. The positive rates of five common pathogens of ordinary PCR and M-PCR were higher than those of bacterial culture (all P < 0.05). The M-PCR had merit for rapid detection compared with ordinary PCR
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|a CONCLUSION: Compared with bacterial culture, ordinary PCR and M-PCR yield higher positive rates in identifying five common pathogens of VAT and VAP, meanwhile, it also demonstrated the tendency that M-PCR may save cost and labor power
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|a Comparative Study
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|a Journal Article
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|a Research Support, U.S. Gov't, Non-P.H.S.
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| 700 |
1 |
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|a Dong, Chen-ming
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Zhao-hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Hong-song
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Mou, Cheng-hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Hong
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 24(2012), 7 vom: 06. Juli, Seite 423-6
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
8 |
|g volume:24
|g year:2012
|g number:7
|g day:06
|g month:07
|g pages:423-6
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 24
|j 2012
|e 7
|b 06
|c 07
|h 423-6
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