Electrochemical synthesis of gold and protein gradients on particle surfaces
A straightforward, versatile approach to the production of protein gradients on planar and spherical particle surfaces is described. The method is based on the spatially controlled oxidation of thiolated surfaces by Au(III) ions generated via the electrochemical oxidation of a gold electrode in a ph...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1992. - 28(2012), 28 vom: 17. Juli, Seite 10318-23 |
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| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
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| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Particulate Matter Proteins Sulfhydryl Compounds Gold 7440-57-5 |
| Zusammenfassung: | A straightforward, versatile approach to the production of protein gradients on planar and spherical particle surfaces is described. The method is based on the spatially controlled oxidation of thiolated surfaces by Au(III) ions generated via the electrochemical oxidation of a gold electrode in a phosphate-buffered saline solution (10 mM PBS, pH 7.2, 150 mM NaCl). Because the gold electrode is in direct contact with the thiolated surfaces, the released Au(III) ions, which are present as Au(III) chloride complexes, give rise to the formation of a surface gradient of Au(I)-thiolate complexes depending on the local redox potential given by the local Au(III) concentration. As is shown on the basis of the use of X-ray photoelectron spectroscopy and fluorescently labeled proteins, the Au(I)-thiolate complexes can subsequently be functionalized with thiolated proteins, yielding surface density protein gradients on micrometer-sized nonconducting polymer beads as well as linear Au(I)-thiolate gradients on planar silicon surfaces |
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| Beschreibung: | Date Completed 31.12.2012 Date Revised 17.07.2012 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la3017694 |