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DE-627 |
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20250214030445.0 |
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231224s2012 xx ||||| 00| ||chi c |
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|a pubmed25n0728.xml
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|a (DE-627)NLM218512473
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|a (NLM)22685717
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a chi
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| 100 |
1 |
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|a Wang, Zhong-hua
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Expression and effects of microRNA-155 in the livers of septic mice
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| 264 |
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1 |
|c 2012
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 21.02.2013
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|a Date Revised 11.06.2012
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a OBJECTIVE: To evaluate the effects of microRNA-155 (miR-155) on liver injury in mice with sepsis
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| 520 |
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|a METHODS: One hundred and twenty BALB/c mice were randomly divided into two groups of equal number according to random number table. Sepsis was induced by intraperitoneal injection of lipopolysaccharide (LPS,20 mg/kg). The mice were sacrificed at the time-points of 0, 2, 6, 12, 24, 48 hours. Blood and liver tissue were collected, and the levels of tumor necrosis factor- α (TNF- α ), interleukin (IL-6, IL-10) in serum and liver homogenate and alanine transaminase (ALT) in serum were determined by enzyme linked immunosorbent assay(ELISA). The injury of liver tissue was evaluated by histopathology. The expression of miR-155 in liver tissue was assessed by fluorescent quantitation reverse transcription-polymerase chain reaction (RT-PCR)
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| 520 |
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|a RESULTS: The levels of TNF- α , IL-6 and IL-10 in serum and liver homogenate of septic mice increased with passage of time, and then the levels of TNF-α and IL-6 lowered after reaching the peak value, but remained higher than that of control group.TNF-α (ng/L) reached the peak value at 2 hours post-LPS-injection (serum: 1538.46 ± 102.12 vs. 64.52 ± 18.44,liver homogenate: 255.26 ± 41.23 vs. 60.21 + 13.55, both P<0.05). The level of IL-6 (μg/L) reached the peak value at 6 hours post-LPS-injection (serum: 875.33 ± 102.37 vs. 153.72 ± 20.67, liver homogenate: 9.22 + 0.82 vs. 3.35 ±0.36, both P<0.05), and that of IL-10 (ng/L) reached the peak value at 48 hours post-LPS-injection (serum: 520.13 ± 88.52 vs. 23.43 3.01, liver homogenate: 260.12 + 50.38 vs. 16.37 ± 3.71, both P<0.05). There were significant differences in above indexes between septic and control group (all P<0.05). The serum level of ALT (U/L) rose with passage of time, reaching the peak value at 48 hours post-LPS-injection (603.26 + 70.21 vs. 45.84 + 5.64, P<0.05). The values showed significant differences between septic and control group (P<0.05). A large number of leucocytic infiltration was found in liver. Hepatic tissue showed architectural distortion. Hepatocyte vacuolation and nodular necrosis were obvious at 12 hours post-LPS-injection. Relative expression of miR-155 was found to be increased at 2 hours post-LPS-injection, reaching its peak value at 12 hours post-LPS-injection [(72.96 ± 9.34)-fold of control group, P<0.05]
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| 520 |
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|a CONCLUSION: The increase in miR-155 expression might play an important role in the mechanism of liver injury during sepsis
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|a English Abstract
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| 650 |
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4 |
|a Journal Article
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| 650 |
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|a MicroRNAs
|2 NLM
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| 650 |
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7 |
|a Mirn155 microRNA, mouse
|2 NLM
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| 700 |
1 |
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|a Liang, Yan-bing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tang, Hao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Zhi-bin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Zhen-yu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Jing-guo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Qing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zeng, Li-jin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hu, Xu-chu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ma, Zhong-fu
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue
|d 1998
|g 24(2012), 3 vom: 01. März, Seite 154-7
|w (DE-627)NLM098227793
|x 1003-0603
|7 nnns
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| 773 |
1 |
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|g volume:24
|g year:2012
|g number:3
|g day:01
|g month:03
|g pages:154-7
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| 912 |
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|a GBV_USEFLAG_A
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| 912 |
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|a SYSFLAG_A
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| 912 |
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|a GBV_NLM
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| 912 |
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|a GBV_ILN_350
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| 951 |
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|a AR
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| 952 |
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|d 24
|j 2012
|e 3
|b 01
|c 03
|h 154-7
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