|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM218384459 |
| 003 |
DE-627 |
| 005 |
20231224040313.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231224s2012 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1111/j.1469-8137.2012.04196.x
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n0728.xml
|
| 035 |
|
|
|a (DE-627)NLM218384459
|
| 035 |
|
|
|a (NLM)22670626
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Vuttipongchaikij, Supachai
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Arabidopsis GT34 family contains five xyloglucan α-1,6-xylosyltransferases
|
| 264 |
|
1 |
|c 2012
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Completed 02.11.2012
|
| 500 |
|
|
|a Date Revised 21.03.2022
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status MEDLINE
|
| 520 |
|
|
|a © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.
|
| 520 |
|
|
|a The Arabidopsis genome includes seven family 34 glycosyltransferase (GT34) encoding genes. XXT1 and XXT2 have previously been shown to encode XyG α-1,6-xylosyltransferases, while knockout mutants of a third, XXT5, exhibit decreased XyG content, suggesting a similar activity. Here, we extend the study to the rest of the Arabidopsis GT34 genes in terms of biochemical activity and their roles in XyG biosynthesis. The enzyme activity of XXTs was investigated using recombinant protein expressed in E. coli. XyG analysis of single and double T-DNA insertion knockouts, together with overexpression of GT34s in selected mutant lines, provided detailed function of each gene. We reveal the activity of the third member of the GT34 gene family (XXT4) that exhibits xylosyltransferase activity. Double mutants for either xxt2 or xxt5 had a large impact on XyG content, structure and size distribution. Overexpression of the remaining member, XXT3, was able to restore XyG epitopes in xxt2, xxt5 and xxt2 xxt5 double knockouts, suggesting that it also encodes a protein with XXT activity. Our work demonstrates that five of the seven Arabidopsis GT34 genes encode XXT enzymes
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Research Support, Non-U.S. Gov't
|
| 650 |
|
7 |
|a Arabidopsis Proteins
|2 NLM
|
| 650 |
|
7 |
|a Epitopes
|2 NLM
|
| 650 |
|
7 |
|a Glucans
|2 NLM
|
| 650 |
|
7 |
|a Recombinant Fusion Proteins
|2 NLM
|
| 650 |
|
7 |
|a Xylans
|2 NLM
|
| 650 |
|
7 |
|a xyloglucan
|2 NLM
|
| 650 |
|
7 |
|a 37294-28-3
|2 NLM
|
| 650 |
|
7 |
|a Pentosyltransferases
|2 NLM
|
| 650 |
|
7 |
|a EC 2.4.2.-
|2 NLM
|
| 650 |
|
7 |
|a xyloglucan xylosyltransferase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.4.2.-
|2 NLM
|
| 700 |
1 |
|
|a Brocklehurst, David
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Steele-King, Clare
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Ashford, David A
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Gomez, Leonardo D
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a McQueen-Mason, Simon J
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 195(2012), 3 vom: 02. Aug., Seite 585-595
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
|
| 773 |
1 |
8 |
|g volume:195
|g year:2012
|g number:3
|g day:02
|g month:08
|g pages:585-595
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1111/j.1469-8137.2012.04196.x
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 195
|j 2012
|e 3
|b 02
|c 08
|h 585-595
|