In situ monitoring of protein adsorption on a nanoparticulated gold film by attenuated total reflection surface-enhanced infrared absorption spectroscopy
In situ surface enhanced infrared absorption spectroscopy (SEIRAS) with an attenuated total reflection (ATR) configuration has been used to monitor the adsorption kinetics of bovine hemoglobin (BHb) on a Au nanoparticle (NP) film. The IR absorbance for BHb molecules on a gold nanoparticle film depos...
| Veröffentlicht in: | Langmuir : the ACS journal of surfaces and colloids. - 1999. - 28(2012), 25 vom: 26. Juni, Seite 9460-5 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2012
|
| Zugriff auf das übergeordnete Werk: | Langmuir : the ACS journal of surfaces and colloids |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Hemoglobins Gold 7440-57-5 Silicon Z4152N8IUI |
| Zusammenfassung: | In situ surface enhanced infrared absorption spectroscopy (SEIRAS) with an attenuated total reflection (ATR) configuration has been used to monitor the adsorption kinetics of bovine hemoglobin (BHb) on a Au nanoparticle (NP) film. The IR absorbance for BHb molecules on a gold nanoparticle film deposited on a Si hemispherical optical window is about 58 times higher than that on a bare Si optical window and the detection sensitivity has been improved by 3 orders of magnitude. From the IR signal as a function of adsorption time, the adsorption kinetics and thermodynamics can be explored in situ. It is found that both the electrostatic interaction and the coordination bonds between BHb residues and Au NP film surface affect the adsorption kinetics. The maximum adsorption can be obtained in solution pH 7.0 (close to the isoelectric point of the protein) due to the electrostatic interaction among proteins. In addition, the isotherm of BHb adsorption follows well the Freundlich adsorption model |
|---|---|
| Beschreibung: | Date Completed 31.10.2012 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/la300819u |